Sterile Forceps Transplantation

Sterile forceps transplantation is an aseptic technique for transferring biological material between containers, tissues, or experimental systems while minimizing exposure to microorganisms and environmental contaminants. The procedure relies on sterilized forceps, controlled handling, and rapid transfer to preserve sample integrity and prevent unintended microbial introduction. In immunology and infection research, this approach supports the movement of tissues, microbial communities, or experimental specimens under defined conditions, helping researchers distinguish effects caused by the transplanted material from those caused by contamination. Consistent aseptic transplantation improves reproducibility and strengthens studies of host–microbe interactions, immune responses, colonization, and infectious disease mechanisms.

Sterile Forceps Transplantation - Related Videos

Research

JoVE Journal - Medicine
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Calibrated Forceps Model of Spinal Cord Compression Injury

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Cited by 57 •

2015

Spinal cord injury models should be highly reproducible. We demonstrate that the calibrated forceps compression model of spinal cord injury is an easy to use surgical method for generating reproducible injuries to the murine spinal cord.

Education

JoVE Science Education - Basic Biology

Sterile Tissue Harvest

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2016

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...

Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology

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2026

This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.

A Modified Method for Heterotopic Mouse Heart Transplantion

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Cited by 5 •

2014

A technique is demonstrated for the microsurgical procedure for heterotopic transplantation of hearts in mice, including simplified methods for donor harvesting and recipient vessel anastomosis.

Research

JoVE Journal - Medicine
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Orthotopic Left Lung Transplantation in Rats

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Cited by 1 •

2025

This protocol describes an improved technique for orthotopic left lung transplantation in rats, focusing on reducing operational difficulty and improving survival rates.

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