Target Cell Labeling
Target Cell Labeling - Related Videos
08:26
Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
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Cited by 22 •
2015
Targeted cell delivery is useful in a variety of biomedical applications. The goal of this protocol is to use superparamagnetic iron oxide nanoparticles (SPION) to label cells and thereby enable magnetic cell targeting approaches for a high degree of control over cell delivery and localization.
09:49
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
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Cited by 38 •
2010
Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.
11:34
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
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Cited by 3 •
2017
Following the preparation of a 64Cu-modified monoclonal antibody binding to a transgenic murine T cell receptor, T cells are radiolabeled in vivo, analyzed for viability, functionality, labeling stability and apoptosis, and adoptively transferred into mice with an airway delayed-type hypersensitivity reaction for non-invasive imaging by positron emission tomography/computed tomography (PET/CT).
13:13
Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
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Cited by 5 •
2021
Here, we present a method for investigating neurite morphogenesis in postnatal mouse retinal explants by time-lapse confocal microscopy. We describe an approach for sparse labeling and acquisition of retinal cell types and their fine processes using recombinant adeno-associated virus vectors that express membrane-targeted fluorescent proteins in a Cre-dependent manner.
10:54
Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
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Cited by 7 •
2017
Here, we describe a protocol for radiolabeling and in vivo testing of tridentate 99mTc(I) chelate-tetrazine derivatives for pre-targeting and bioorthogonal chemistry.
FAQs
The label’s location determines what can be observed. A probe associated with the target-cell membrane can help distinguish those cells from lymphocytes, whereas an intracellular label can report changes occurring within the target. This distinction matters when interpreting signals, because immune attack may produce target-cell death, uptake by another cell, or loss of membrane integrity.
Fluorescent dyes, antibody-linked probes, and radioactive tracers provide different signal formats rather than identical measurements. Fluorescent labels support flow cytometry and microscopy, antibody-linked probes can mark selected cellular features, and radioactive tracers provide a measurable tracer signal. The choice should match whether the experiment emphasizes cell identification, tracking, or quantifying target-cell damage.
In cytotoxicity experiments, labeling creates a reference population that can be separated from natural killer cells or cytotoxic T cells during analysis. After immune effectors interact with the targets, investigators can examine label-related evidence of death, uptake, or membrane-integrity loss. This enables comparisons of effector activity across conditions instead of relying only on visual inspection.
Flow cytometry and microscopy extract different kinds of information from labeled targets. Flow cytometry supports measurement across distinguishable cell populations, while microscopy allows researchers to observe labeled cells and their interactions directly. With either approach, the label connects an immune response to a defined target population, which is important when lymphocytes and targets occur together.
A cytotoxicity experiment generally labels the target-cell population first, establishes a way to distinguish those cells from lymphocytes, and then assesses changes after immune-effecter contact. Researchers can measure the resulting signal with flow cytometry, microscopy, or another tracer-based readout. The outcome is evidence of target-cell damage, death, uptake, or membrane-integrity loss.
In immunology, labeled targets support assays that quantify natural killer cell and cytotoxic T-cell activity. In infection studies, the approach helps evaluate damage caused by pathogens and compare immune responses under different experimental conditions. Because the same labeled population can be tracked or measured, researchers can relate cellular injury to a defined immune or infectious context.