Viral Capsid Selection
Viral Capsid Selection - Related Videos
12:57
Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
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Cited by 6 •
2017
Routine detection methods utilizing viral genome amplification are limited by their inability to discriminate infectious from non-infectious particles. The purpose of this article is to provide detailed protocols for alternative methods to aid in discrimination of infectious norovirus particles using aptamer binding, dynamic light scattering, and transmission electron microscopy.
03:24
Visualizing Heat-Treated Viral Capsids Using Transmission Electron Microscopy
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2026
Source: Moore, M. D., et al. Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity. J. Vis. Exp.(2017).This video demonstrates how purified viral capsids are heat-treated, prepared, and imaged using transmission electron microscopy (TEM) to reveal structural changes and heat-induced damage.
07:24
In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation
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Cited by 4 •
2016
Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.
13:36
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
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Cited by 1 •
2015
Here, we present a protocol to generate a proof-of-principle divalent adenovirus type 5 (Ad5) vector Ad5/H5-HVR1-KWAS-HVR5-His6 by utilizing the Antigen Capsid-Incorporation strategy. This vector was demonstrated to exhibit qualitative fitness, the capability to escape Ad5-positive sera in vitro, and the antigenicity as well as immunogenicity to the incorporated antigens.
00:56
Viral Structure
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2019
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid. Structural Classes of Viruses Many criteria are used to classify viruses, including capsid design. Most viruses...
FAQs
Surface proteins on a capsid influence which cellular receptors a viral vector can bind, providing a basis for comparing tropism. Tropism describes the pattern of cells or tissues that may be engaged. Assessing receptor interactions therefore helps identify capsids suited to particular delivery goals and infection studies.
Viral capsid selection compares candidates by examining whether they remain stable under conditions relevant to the intended use and support genome delivery. These criteria matter because a capsid may be attractive for cellular entry yet unsuitable if its stability or delivery performance does not match the application. Considering both properties helps narrow candidates before assessing immune interactions.
Antibody interactions can change the suitability of a capsid even when cellular entry is effective. Comparing how capsids interact with antibodies and other immune components helps researchers assess antigenicity and susceptibility to neutralization. This information is important when selecting vectors or interpreting infection and vaccine studies.
A practical comparison starts by defining the biological or therapeutic purpose. Candidates, whether natural or engineered, are then assessed for surface-protein receptor binding, genome delivery, stability under relevant conditions, and interactions with antibodies or other immune components. Reviewing these criteria together links a capsid's molecular properties to the intended outcome and supports reasoned candidate selection.
In immunology and infection research, capsid comparisons help characterize viral tropism, meaning the pattern of cellular targeting, alongside antigenicity and susceptibility to neutralization. These outputs connect capsid behavior with infection biology and immune recognition. They can guide studies asking which cells are engaged and how immune components may affect that interaction.
For vaccine development, capsid selection can focus attention on antigenicity and immune recognition, while gene-delivery applications emphasize receptor binding, genome delivery, and stability. The same comparison framework therefore serves different goals: one supports evaluation of immune-facing properties, and the other helps identify vectors with suitable entry and delivery characteristics under intended conditions.