Graft Preservation
Graft Preservation - Related Videos
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
02:34
Modeling Preservation Injury in Porcine Donor Kidney: A Procedure to Induce Graft Dysfunction by Subjecting Kidney to Prolonged Cold Ischemia
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2025
In this video, we describe a technique to induce preservation injury via the prolonged cold ischemic storage time in porcine donor kidney.
08:15
Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
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2025
Donor heart preservation is a major determinant of success in heart transplantation. Technological advances have led to optimizing the conditions of preservation, along with a more accurate assessment of the donor heart before transplantation. We herein describe two innovative technologies currently applied for cardiac preservation: uniform cooling preservation and normothermic ex-situ heart perfusion.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
03:35
A Technique for Human Skin Grafting in a Mouse Model
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2025
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.
FAQs
Controlled cooling lowers cellular metabolism, which helps reduce the rate of biochemical changes while the graft is outside the body. By slowing these processes, cooling can limit ischemic injury and help maintain tissue or organ function during the interval between retrieval, transport, and transplantation. The approach is therefore central when immediate implantation is not possible.
Specialized preservation solutions support graft storage by helping limit edema and biochemical changes during the preservation interval. Their use complements controlled cooling rather than replacing it, addressing cellular conditions that can worsen while blood flow is interrupted. Together, the solution and temperature-management strategy help maintain the graft in a condition suitable for later transplantation.
Machine perfusion adds a fluid-delivery process to graft preservation by circulating, and in some approaches oxygenating, fluid through the graft. This can provide more active support than cooling and solution exposure alone. It may also allow assessment of graft condition, giving the transplant team additional information when determining whether a donated organ is suitable for transplantation.
After retrieval, preservation methods are applied while the graft awaits transport, compatibility testing, surgical preparation, and implantation. Controlled cooling and preservation solutions help limit injury during this interval, while machine perfusion may provide oxygenated fluid and condition assessment. The specific preservation approach determines how the graft is supported as it moves toward transplantation.
Machine perfusion may be considered when preservation requires more than passive cooling and solution-based storage, particularly when information about graft condition could support clinical decision-making. Oxygenated fluid delivery and condition assessment are important features of this approach. Its potential value lies in helping teams evaluate and preserve donated organs during the preparation period before transplantation.
Effective graft preservation extends the time available for transportation, compatibility testing, and surgical preparation without abandoning efforts to maintain graft viability and function. This additional time can improve coordination across the transplant process and help support transplant outcomes. Preservation advances may also make it possible to use donated organs or tissues that would otherwise be unsuitable for transplantation.