Irdye 800cw-egf
Irdye 800cw-egf - Related Videos
04:13
A Technique to Assess the In Vivo Localization of Tumor-Specific Antibodies
0 Views •
2025
This video demonstrates a technique to study the in vivo localization of tumor-specific antibodies in a mouse tumor xenograft model. Upon subcutaneously injecting cancer cells into mice for tumor formation, fluorescently-labeled antibodies are injected that bind to tumor-specific receptors, enabling in vivo assessment of antibody localization via imaging.
12:55
Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
0 Views •
Cited by 7 •
2008
We demonstrate that the over expression of epidermal growth factor receptors (EGFR) enhances the motility of neural stem cells(NSCs) using a novel agarose gel based microfluidic device. This technology can be readily adaptable to other mammalian cell systems where cell sources are scarce, such as human neural stem cells, and the turn around time is critical.
04:51
Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
0 Views •
Cited by 12 •
2009
HC11 lactogenic differentiation can be characterized by the formation of domed structures referred to as mammospheres. The structures can be enumerated by phase contrast microscopy to aid in quantifying lactogenic differentiation.
11:05
A Gradient-generating Microfluidic Device for Cell Biology
0 Views •
Cited by 14 •
2007
We describe a protocol for the microfabrication of the gradient-generating microfluidic device that can generate spatial and temporal gradients in well-defined microenvironment. In this approach, the gradient-generating microfluidic device can be used to study directed cell migration, embryogenesis, wound healing, and cancer metastasis.
07:26
Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
0 Views •
Cited by 47 •
2012
An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
FAQs
The EGF component recognizes EGFR on the cell surface, linking receptor presence with fluorescence. After binding, receptor-mediated uptake can concentrate the conjugate inside responsive cells, potentially strengthening the observable signal relative to areas with less receptor engagement. This relationship allows investigators to examine receptor distribution while considering both surface binding and intracellular accumulation when interpreting near-infrared images.
Uptake adds a cellular process beyond initial receptor binding. A fluorescent pattern may reflect where EGFR is present and where receptor engagement leads to internal concentration of the conjugate. Consequently, image intensity should be considered as an indicator of probe interaction and uptake within a biological model, rather than treated as a direct measurement of receptor abundance alone.
The IRDye 800CW fluorophore produces a signal that can be detected with near-infrared imaging systems. This provides an observable readout for comparing fluorescent patterns across cells or tissues in research models. In medicine-focused studies, the optical signal helps connect molecular interactions at EGFR with spatial information about receptor distribution, tumor biology, or changes associated with treatment response.
A cultured-cell workflow can compare fluorescent signal among cells with differing EGFR expression or responsiveness. Investigators expose the cells to the conjugate, detect the resulting near-infrared fluorescence, and examine its cellular distribution. Comparing signal patterns with receptor-related characteristics can help determine whether observed fluorescence is consistent with EGFR binding and receptor-mediated uptake.
IRDye 800CW-EGF can support examination of how EGFR-related activity is distributed across tumor cells or tissues. Fluorescent patterns may reveal areas with differing receptor engagement or probe accumulation, giving researchers a way to study tumor biology spatially. These observations can complement broader investigations of receptor behavior and help assess molecular features relevant to targeted imaging.
In animal models or clinical research workflows, the conjugate can serve as a molecular imaging readout for EGFR-associated patterns. Near-infrared detection enables investigators to observe where the probe localizes within the studied system and compare those patterns across experimental conditions. Its role is investigational, supporting assessment of receptor distribution, targeted imaging strategies, and treatment-related changes.
Researchers can compare near-infrared fluorescence patterns before or after an intervention to investigate whether EGFR-associated localization or uptake changes with treatment. Such comparisons may provide molecular context for treatment response studies, especially when signal distribution differs among cells or tissues. The probe therefore links an observable imaging outcome with receptor-related behavior rather than relying only on structural observations.