Leukocyte Imaging
Leukocyte Imaging - Related Videos
07:42
Non-invasive Imaging of Leukocyte Homing and Migration in vivo
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Cited by 8 •
2010
Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
10:10
In Vivo Imaging Uncovers the Migratory Behavior of Leukocytes within the Joints
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2025
In vivo imaging of the joints is a valuable technique for uncovering the migratory behavior of leukocytes and molecular dynamics within the inflamed joints. Here, we present a detailed protocol for in vivo imaging of the joints using a murine model of type II collagen-induced arthritis (CIA).
06:20
Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
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Cited by 22 •
2011
This is a method to visualise leukocyte adhesion to the endothelium in harvested pressurised vessels. The technique enables studying vascular adhesion under shear flow with differing intraluminal pressures up to 200 mmHg thus mimic-ing the pathophysiological conditions of high blood pressure.
05:12
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
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Cited by 11 •
2015
This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
FAQs
Identification depends on the imaging platform. Some systems use natural cellular contrast, whereas others use fluorescent labels that bind leukocyte surface markers. This distinction affects how confidently cells can be recognized within blood, tissues, or living organisms. Marker-based visualization can also support analysis of particular cell populations when surface characteristics are relevant to the study.
Leukocyte imaging can reveal dynamic behaviors, including adhesion, migration, infiltration, and direct cell-cell contact. These observations show how immune cells move toward affected sites and interact with surrounding cells. Examining behavior rather than morphology alone helps connect cellular activity with local immune coordination, inflammation, infection, immune disorders, or cancer-related processes.
Quantitative analysis converts visual observations into measurable information about leukocyte distribution, movement, and interactions. This added measurement helps investigators compare cellular behavior across conditions, assess how immune cells reach affected sites, and examine changes associated with disease progression or treatment. Imaging therefore contributes both descriptive evidence and a basis for evaluating biological differences.
A typical workflow selects an appropriate source, such as blood, tissue, or a living organism, then establishes how leukocytes will be identified using natural contrast or fluorescent labels. Microscopy captures the relevant cells and behaviors, after which image analysis evaluates morphology, distribution, movement, or interactions. The chosen sequence should match the biological question.
Researchers can apply Leukocyte Imaging to investigate inflammation, infection, immune disorders, and cancer. The approach is particularly useful when the research question concerns where leukocytes are located, how they move, whether they enter affected tissues, or how they contact other cells. These observations provide cellular context for understanding local immune responses.
Changes in leukocyte morphology, distribution, movement, infiltration, or cell-cell contact can provide evidence of altered immune activity. In medical studies, these measurements may help evaluate disease progression or treatment response by showing how immune-cell behavior changes over time or under different conditions. Interpreting several imaging features together gives a broader view of the local response.