Podocyte Marker Proteins
Podocyte Marker Proteins - Related Videos
04:19
Deriving Human Kidney Podocytes from Induced Pluripotent Stem Cells: A Procedure for Directed Differentiation of Mature Kidney Podocytes from Stem Cells
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2023
In this video, we describe the protocol to derive human kidney podocytes from induced pluripotent stem cells through cell differentiation under defined chemical conditions. The generated podocytes can be used for nephrotoxicity testing and disease modeling.
12:19
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Cited by 25 •
2015
Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.
06:18
Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
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Cited by 3 •
2021
The presented method enables visualization of fluorescently labeled cellular proteins with expansion microscopy leading to a resolution of 70 nm on a conventional microscope.
08:52
Generation of Patient-Derived Podocytes from Skin Biopsies
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Cited by 5 •
2023
This manuscript describes a two-step protocol to generate patient-specific podocytes from dermal fibroblasts via episomal reprogramming into human-induced pluripotent stem cells (hiPSCs) and subsequent differentiation into podocytes.
05:44
The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
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Cited by 44 •
2016
Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.
FAQs
Their locations create complementary readouts: nephrin and podocin indicate the slit diaphragm, synaptopodin reflects the actin cytoskeleton, and WT1 reports nuclear features. Examining these signals together can connect molecular changes with podocyte structure and differentiation rather than treating a single protein as a complete description. This combined view is useful when interpreting glomerular injury.
Distribution links each protein to a particular podocyte compartment and function. Signals at the slit diaphragm, within the actin cytoskeleton, or in the nucleus can therefore reflect different aspects of cellular organization and differentiation. Changes in where markers appear, as well as how strongly they are expressed, may provide a more informative assessment of podocyte status.
Changes in marker expression or distribution can indicate podocyte development, injury, or loss, and may reflect altered filtration-barrier function. These findings help researchers relate molecular observations to disturbances in the glomerulus. They are especially relevant when studying conditions in which filtration is impaired, including proteinuric disorders.
Researchers can examine these proteins through immunostaining, immunoblotting, or gene-expression methods. Together, these approaches support assessment of marker presence, expression, and distribution, depending on the study design. The resulting data can be used to evaluate podocyte differentiation, injury, loss, and changes associated with glomerular filtration-barrier function.
They are relevant when investigators study glomerular diseases, particularly proteinuric disorders, where podocyte condition and filtration-barrier function are important concerns. Marker analysis can help characterize disease-associated changes and support diagnostic investigation. It also provides a molecular way to examine how podocyte abnormalities relate to kidney damage.
Marker expression and distribution can be examined before and after an experimental treatment to assess whether podocyte-related changes are altered. Because the markers reflect structure, differentiation, injury, loss, or filtration-barrier function, they can provide evidence about treatment-associated effects. This makes them useful in studies of therapies targeting glomerular or kidney damage.