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The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
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The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

DOI:
10.3791/53613-v

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05:44 min

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April 25, 2016

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, , , , , ,
1Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363,University Paris 13, 2UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique,Sorbonne Universités, 3Laboratory of Excellence GR-Ex, 4Laboratory MOVE (EA 6314),University of Poitiers

Chapters

  • 00:05Title
  • 00:44Day 1 Endogenous Peroxidase Activity Destruction, Non-specific Binding Site Blockade and Incubation with the Primary Antibody
  • 01:44Day 3 Incubation with Secondary Antibody and Development of a Color Reaction
  • 03:21Sample Mounting
  • 04:16Results: c-FOS-positive Neurons on Sections of the Medulla Oblongata in Control Mice and Mice Submitted to Hypoxia In Vivo
  • 05:17Conclusion

Summary

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Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.

Tags

C-FOS ProteinImmunohistological DetectionMarkerCentral PathwaysPhysiological ResponsesIn VivoEx VivoNeuronal ActivityBrain MappingBrain RegionsNeurophysiological FieldC-FOS GeneStimulationQuantificationTest SimulationCoronal Brain Stem SectionsWashPBSEndogenous Peroxidase ActivityHydrogen PeroxideBlock Nonspecific Binding SitesGoat SerumPBSTPolyclonal Antibody

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