The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses <em>In Vivo</em> and <em>Ex Vivo</em>

Anne-Sophie Perrin-Terrin; Florine Jeton; Aurelien Pichon; Alain Frugi&#232;re; Jean-Paul Richalet; Laurence Bodineau; Nicolas Voituron

doi:10.3791/53613

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The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses <em>In Vivo</em> and <em>Ex Vivo</em>

JoVE Journal
Biology

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JoVE Journal Biology

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

33,575 Views

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05:44 min

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April 25, 2016

DOI:

10.3791/53613-v

DOI:

10.3791/53613-v

•

05:44 min

April 25, 2016

•

33549 Views

Anne-Sophie Perrin-Terrin^1,2, Florine Jeton^1,3, Aurelien Pichon^1,3,4, Alain Frugière², Jean-Paul Richalet^1,3, Laurence Bodineau², Nicolas Voituron^1,3

¹Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363,University Paris 13 ²UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique,Sorbonne Universités ³Laboratory of Excellence GR-Ex ⁴Laboratory MOVE (EA 6314),University of Poitiers

Summary

Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.