Portal Vein Puncture
Portal Vein Puncture - Related Videos
05:27
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
0 Views •
Cited by 87 •
2007
Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.
05:15
Portal Vein Injection: A Method to Study Cancer Metastasis to the Liver
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2023
This video describes a surgical procedure to deliver mammary tumor cells to the murine liver via portal vein injection. This model permits the investigation of late stages of liver metastasis.
03:18
In Vivo Murine Liver Perfusion: A Method to Infuse the Rodent Liver with Solution via the Portal Vein
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2023
This video describes a procedure for mouse liver perfusion through the portal vein using collagenase solution prior to isolating liver cells.
08:47
3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System
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Cited by 20 •
2012
A method of visualizing and quantifying the 3-dimensional structure of mouse hepatic portal vein or intrahepatic bile duct is described. This resin cast technique can also be applied to other ductal or vascular systems and allows for in situ visualization or quantification of a system's intact communicating architecture.
03:48
Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding
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2025
In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.
FAQs
The transhepatic route provides a direct path toward a portal vein branch through liver tissue, allowing the operator to target the portal venous system from within the liver. This approach is typically paired with ultrasound or fluoroscopic guidance, which helps visualize the intended trajectory during needle advancement and supports controlled access.
Guidance matters because the needle must reach a portal vein branch while limiting contact with nearby vessels and bile ducts. Ultrasound or fluoroscopy supplies imaging during the puncture, allowing the trajectory to be monitored rather than relying on unguided advancement. The practical goal is accurate entry with reduced injury to surrounding structures.
Controlled advancement is central to the technique because access depends on entering a selected portal vein branch, not simply advancing toward the liver. Maintaining control helps balance two objectives: achieving a usable connection with the portal venous system and minimizing unintended injury to adjacent anatomy. This principle also supports subsequent measurements or interventions.
Once access is established, clinicians can measure portal pressure, create portographic images, or collect blood samples. These outputs provide different kinds of information: pressure measurement assesses the portal circulation, portography depicts it, and sampling permits analysis of portal venous blood. The same access can therefore support both evaluation and procedural planning.
Portal vein puncture is relevant when clinicians need direct information about disorders affecting portal flow, particularly portal hypertension and liver disease. Pressure measurements can characterize the portal circulation, while portography can show vascular findings associated with the clinical problem. Together, these capabilities make the technique useful for diagnosis as well as treatment planning.
The puncture can also establish access for selected vascular procedures, including transjugular intrahepatic portosystemic shunt placement. In this setting, the technique serves as the access point needed to proceed with the intervention rather than only providing measurements or samples. It may also assist management of selected vascular disorders when portal venous entry is required.