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Chapters
- 00:05Title
- 01:09Prepare Cannula and Syringe with PBS
- 02:13Prepare Mouse and Cannulate Extrahepatic Portal Vein or Common Bile Duct
- 04:40Resin Cast the Portal Vein or Intrahepatic Bile Duct
- 05:42Clear Liver for Visualization in situ
- 07:24Representative Liver Resin Casting
- 08:25Conclusion
A method of visualizing and quantifying the 3-dimensional structure of mouse hepatic portal vein or intrahepatic bile duct is described. This resin cast technique can also be applied to other ductal or vascular systems and allows for in situ visualization or quantification of a system's intact communicating architecture.
Tags
3-DimensionalResin CastingImagingMouse Portal VeinIntrahepatic Bile Duct SystemArchitectureVascular StructuresDuctal StructuresPhysiological FunctionFormationMaintenanceHighly Regulated ProcessAnalysis2-dimensional ExaminationSectionDye Injection StudiesComplete RepresentationQuantifiable RepresentationNovel TechniqueVisualizing StructuresQuantifying StructuresPlastic Resin CastsPermanent SnapshotConnected StructureCommunicating StructureBlood VesselDuct NetworkOrgan FunctionMorphological ChangesDisease States
