Blood Oxygenation
Blood Oxygenation - Related Videos
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 28 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
01:27
Oxygen Transport in the Blood
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2024
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
03:46
Blood Oxygen Level-Dependent Functional Magnetic Resonance Imaging of the Visual Cortex
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2025
Source: Galenchik-Chan, A., et al. Functional Magnetic Resonance Imaging (fMRI) of the Visual Cortex with Wide-View Retinotopic Stimulation. J. Vis. Exp. (2023). The video demonstrates functional magnetic resonance imaging (fMRI) of the visual cortex to map activity based on blood oxygenation levels. Structural MRI images are acquired as anatomical references, followed by fMRI scans. Visual stimuli are displayed to activate retinotopic neurons in the visual cortex. The increased energy demand...
10:40
Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model
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Cited by 10 •
2012
An optical system was developed to visualize hepatic microcirculation with FITC-labeled erythrocytes and to measure the partial pressure of oxygen in the microvessels with laser-assisted phosphorimetry. This method can be used to investigate physiological and pathological mechanisms by analyzing microvascular structure, diameter, blood flow velocity, and oxygen tension.
08:56
Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
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Cited by 46 •
2015
Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.
FAQs
Reversible binding allows hemoglobin to load oxygen in the lungs and release it where oxygen levels are lower. This controlled exchange links oxygen acquisition with tissue delivery rather than permanently retaining oxygen in red blood cells. In neural tissue, the resulting availability supports cellular energy production, making oxygen handling relevant to interpreting activity-related changes in the brain.
Blood moving through capillaries encounters lower oxygen levels, favoring separation of oxygen from hemoglobin. That release makes oxygen available to surrounding tissues, while carbon dioxide moves back toward the lungs for removal. This principle explains how circulating blood connects pulmonary gas exchange with the metabolic needs of tissues, including the brain.
Neurovascular coupling links local neuronal activity with local changes in blood oxygenation and energy demand. As neural tissue becomes active, its need for cellular energy is reflected in oxygen-related vascular changes. This relationship allows measurements of blood oxygenation to serve as a way to investigate brain function while remaining grounded in the tissue’s metabolic requirements.
Functional magnetic resonance imaging uses blood-oxygen-level-dependent, or BOLD, changes to map brain activity. The signal arises from activity-related changes in blood oxygenation, which are connected through neurovascular coupling to local neuronal activity and energy demand. Consequently, fMRI can organize patterns of this physiological response for studies of how the brain functions.
They can support investigations of cognition, neurological disorders, and general brain function. In these settings, researchers examine oxygenation-related changes as indicators linked to local neuronal activity through neurovascular coupling. The resulting measurements connect a physiological blood response with questions about how neural systems operate or are affected by neurological disorders.
Functional magnetic resonance imaging detects changes represented by the BOLD signal, and researchers interpret those changes through neurovascular coupling. This framework relates local blood oxygenation to neuronal activity and energy demand. The measured pattern can then be used to map brain activity and support studies of cognition, neurological disorders, or broader brain function.