Field Epsp Recording
Field Epsp Recording - Related Videos
05:26
Recording Neuronal Field Excitatory Postsynaptic Potentials in Acute Hippocampal Slices
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2025
This video demonstrates the procedure for setting up a brain slice in a submersion chamber to record synaptic activity. The process involves securing the slice on lens paper, maintaining a continuous flow of artificial cerebrospinal fluid (aCSF) to ensure neuronal viability, and accurately positioning the stimulation and recording electrodes relative to a specific brain region of interest. This setup allows for precise monitoring of baseline synaptic activity and the effects of electrical...
03:00
Recording of Local Field Potential from Both Hemispheres of the Mouse Brain
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2025
This video demonstrates in vivo recording of local field potential (LFP) from bilateral M2 areas of the mouse brain. An anesthetized mouse is secured and prepared for the procedure. To record LFPs, insert microelectrodes into the target coordinates of the bilateral M2 areas, which are responsible for complex motor movements.
04:17
Concurrent Electroencephalographic and Local Field Potential Recordings in an Anesthetized Rat
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2025
This video demonstrates the procedure for concurrent recordings of co-localized electroencephalography (EEG) and local field potentials (LFPs) in an anesthetized rat. The process involves preparing the rat and attaching stimulating electrodes to its whisker pads. An EEG spider electrode is placed on the rat's skull for surface EEG measurements. At the same time, a multi-channel microelectrode is inserted into a specific brain region through a burr hole in the skull to record neural activity...
02:44
Recording of Local Field Potential in Mouse Hippocampal-Entorhinal Cortex Slices
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2025
The video demonstrates a procedure to record the local field potential (LFP) in mouse hippocampal-entorhinal cortex slices. Electrical stimulation is provided at the CA3 stratum radiatum of the hippocampus, which causes changes in the postsynaptic potential at the CA1 stratum pyramidale. The combined change in the membrane potential of CA1 neurons, termed the local field potential, is recorded.
03:10
Recording CA3-CA1 Synaptic Responses in a Rat Hippocampal Slice
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2025
This video demonstrates a method to record CA3-CA1 synaptic responses in a rat hippocampal slice. It outlines the steps involved in placing the stimulating and recording electrodes on the hippocampal slice and measuring the field excitatory postsynaptic potential (fEPSP) to assess synaptic response strength.
FAQs
The experiment links two events: stimulation of an afferent pathway activates many presynaptic fibers, and an extracellular electrode detects the voltage change in a downstream region. Because the signal represents responses from a neuronal population, its slope or amplitude provides a readout of the strength of synaptic communication in that circuit.
Response slope or amplitude serves as an experimental index of synaptic strength. Measuring these features lets researchers compare the magnitude of evoked population responses under different circuit conditions. This makes the recording useful for detecting changes in synaptic transmission, including those associated with plasticity, without requiring the signal to represent a single neuron.
Stimulating an afferent pathway establishes the input that drives the downstream response. Because many presynaptic fibers are activated together, the extracellular electrode captures a population-level voltage change rather than an isolated cellular event. This arrangement helps investigators examine whether connected regions communicate effectively and how strongly that communication appears in the recorded tissue.
Field EPSP recordings can track synaptic plasticity by comparing evoked responses across experimental conditions. A change in response slope or amplitude can indicate altered synaptic strength after neural circuits undergo processes such as long-term potentiation or long-term depression. This makes the method relevant to questions about how brain connections change over time.
A typical experiment begins by stimulating an afferent pathway. The stimulation activates presynaptic fibers, while an extracellular electrode detects the resulting voltage change in a downstream region. Researchers then evaluate the recorded response, commonly using its slope or amplitude as the measure of synaptic strength.
The recorded signal can provide evidence about circuit connectivity, synaptic transmission, and plasticity. In practice, researchers use it to characterize how an afferent input influences a downstream population and to determine whether response strength changes under the conditions being studied. These outcomes support analysis of network function in brain tissue.
Field EPSP recording is used in studies of learning, development, disease, or drug exposure. In each context, investigators can examine whether neural network responses and synaptic strength differ across the condition of interest. Its population-level readout connects changes in recorded physiology with broader questions about circuit adaptation and dysfunction.