T80 Flask
T80 Flask - Related Videos
08:39
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Cited by 4 •
2011
Cells play an instrumental and increasing role in research, and the discovery and development of new therapeutics. With this increasing need for greater number of cells we need more efficient and effective ways for growing and harvesting attachment dependent cells. A Multilayered flask with the right features can serve this purpose.
07:33
In vivo Dual Substrate Bioluminescent Imaging
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Cited by 20 •
2011
Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
06:09
Intrarenal Injection of Escherichia coli in a Rat Model of Pyelonephritis
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Cited by 13 •
2017
This manuscript describes a rat surgical model of pyelonephritis using direct intra-renal infection by Escherichia coli into the renal pelvis. The experimental procedure can be utilized to study the pathogenesis of pyelonephritis as well as the associated inflammation and fibrosis.
08:18
Degassing Liquids with Freeze-Pump-Thaw Cycling
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2023
Source: Laboratory of Dr. Neil Branda — Simon Fraser University Degassing refers to the process by which dissolved gases are removed from a liquid. The presence of dissolved gases such as oxygen or carbon dioxide can impede chemical reactions that utilize sensitive reagents, interfere with spectroscopic measurements, or can induce unwanted bubble formation. A number of different techniques are available for degassing liquids; some of these include heating, ultrasonic agitation, chemical...
05:39
Shock Wave Application to Cell Cultures
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Cited by 22 •
2014
Shock waves nowadays are well known for their regenerative effects. Therefore in vitro experiments are of increasing interest. We therefore developed a model for in vitro shock wave trials (IVSWT) that enables us to mimic in vivo conditions thereby avoiding distracting physical effects.
FAQs
The treated polystyrene surface promotes attachment of adherent neuronal, glial, or neural progenitor cells. Once cells remain attached, the culture medium can support nutrient exchange and metabolism, while controlled temperature, humidity, and carbon dioxide help maintain the environment needed for proliferation. This attachment is central to obtaining a stable culture for later neuroscience experiments.
Temperature, humidity, carbon dioxide, and culture medium each contribute to cell maintenance and expansion. The medium supports nutrient exchange and metabolism, while the surrounding physical conditions help sustain the culture environment. Changes in any of these factors can affect proliferation and the readiness of neuronal, glial, or neural progenitor cultures for downstream neuroscience experiments.
Its larger growth surface provides more capacity for maintaining and expanding adherent cultures than smaller vessels. This can be useful when experiments require sufficient neuronal, glial, or neural progenitor material for subsequent analyses or treatments. The format supports scale-up of cell preparation while retaining the controlled culture conditions needed for laboratory work.
A basic workflow begins by introducing adherent cells into the treated vessel, then supplying culture medium and maintaining the flask under controlled temperature, humidity, and carbon dioxide. The culture is kept to support attachment, nutrient exchange, metabolism, and proliferation. After expansion, the cells can be taken forward into neuroscience experiments such as differentiation or treatment studies.
They are useful when a neuroscience study needs expanded cell cultures before the experimental phase. Neuronal, glial, and neural progenitor cultures grown in this format can support investigations of differentiation, neurotoxicity, synaptic function, disease mechanisms, and responses to pharmacological treatments. The available surface area makes the vessel practical for preparing cultures at greater capacity than smaller formats.
These cultures serve as a preparation stage by supplying cells for downstream examination of differentiation, neurotoxicity, synaptic function, disease mechanisms, or responses to pharmacological treatments. The relevant readout depends on the study, but the flask's role is to maintain and expand the neural culture under controlled conditions before those investigations are performed.