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Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Article DOI: 10.3791/2393-v • 13:22 min • November 20th, 2010
November 20th, 2010 •
1,2, 2, 2, 2
1 Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran, 2Department of Neurosurgery, University of Florida

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This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.

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Isolation Expansion Adult Mouse Neural Stem Cells Neurosphere Assay Putative Murine Brain Reynolds And Weiss Chemically Defined Serum-free Culture System Growth Factor Responsive Precursor Cells Epidermal Growth Factor Basic Fibroblastic Growth Factor Colonies Undifferentiated Cells Neurospheres Subcultured Pool Of Neural Stem Cells Differentiate Three Major Cell Types CNS Neurons Astrocytes Oligodendrocytes Invaluable Tool In Vitro Studies Therapeutic Purposes Video Demonstration Periventricular Region Reproducible Neurosphere Cultures
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