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Bioengineering

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JoVE Journal Bioengineering
Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3
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Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Article DOI: 10.3791/3149-v • 07:27 min • August 17th, 2011
August 17th, 2011 •
1, 1, 1, 1
1Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston

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This video provides a detailed protocol for studying the pharmacological profile of human TRPA1 channels using FlexStation 3. The protocol covers details of cell preparation, dye loading and operation of the microplate reader, FlexStation 3.

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Cell-based Calcium Assay Medium To High Throughput Screening TRP Channel Functions FlexStation 3 Multi-mode Microplate Reader Fluorescence Measurement Multi-well Plates Academic Settings Integrated Fluid Transfer Module Multi-channel Pipetter Fluorescence Changes Ca2+-permeable Ion Channels G-protein Coupled Receptors Intracellular Free Ca2+ Levels Transient Receptor Potential (TRP) Channels Calcium Influx TRPA1 Channel Molecular Sensor Noxious Stimuli HEK293 Cells Ca2+-sensitive Fluorescent Dye Fluo-4 Real-time Fluorescence Changes TRPA1 Agonist TRPA1 Antagonist
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