JoVE Journal
Biology
Biology
È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.
Capitoli
Riepilogo
Enterobacter sp. YSU grows in glucose minimal salts medium. Auxotrophs are generated by transforming it with a transposome which randomly inserts itself into the host genome. Mutants are found by replica plating from complex medium to minimal medium. Interrupted genes are identified by gene rescue and sequencing.