Replica Plating
Replica Plating - Related Videos
09:23
Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures
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Cited by 7 •
2012
We present methods for fabrication of patterned microstructures of vertically aligned carbon nanotubes (CNTs), and their use as master molds for production of polymer microstructures with organized nanoscale surface texture. The CNT forests are densified by condensation of solvent onto the substrate, which significantly increases their packing density and enables self-directed formation of 3D shapes.
09:49
Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala
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Cited by 6 •
2016
This article illustrates how the expression of neurotransmitter receptors can be quantified and the pattern analyzed at synapses with identified pre and postsynaptic elements using a combination of viral transduction of optogenetic tools and the freeze-fracture replica immunolabeling technique.
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
11:58
The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
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Cited by 6 •
2018
Here we describe the Replica Set method, an approach to quantitatively measure C. elegans lifespan/survival and healthspan in a high-throughput and robust manner, thus allowing screening of many conditions without sacrificing data quality. This protocol details the strategy and provides a software tool for analysis of Replica Set data.
13:29
Jet Impinging on an Inclined Plate
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2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The goal of this experiment is to demonstrate how a fluid flow exerts forces on structures by conversion of dynamic pressure into static pressure. To this end, we will make a plane jet impinge on a flat plate and will measure the resulting pressure distribution along the plate. The resultant force will be estimated by integrating the product between the...
FAQs
The key advantage is positional continuity: each transferred spot corresponds to a specific colony on the master plate. When equivalent positions are examined on plates containing different media or conditions, researchers can attribute a changed growth pattern to that original cell population rather than to an unrelated colony. This supports direct phenotype comparisons.
Changing the conditions on fresh plates reveals which colonies can grow under a particular requirement or challenge. A colony present on the master plate may fail to appear under a specific nutrient condition, respond differently to an antibiotic, or be affected by another environmental factor. These contrasting outcomes expose differences among otherwise related cell populations.
The method compares how corresponding cell populations behave across defined conditions, using growth or failure to grow as observable phenotypes. Consistent differences can identify populations with genetic traits such as auxotrophy or antibiotic resistance. In this way, a colony’s position and response provide a practical link between an inherited cellular characteristic and its visible laboratory outcome.
Several fresh plates allow the same set of colonies to be tested simultaneously against different nutrients, antibiotics, or environmental conditions. Because the colony positions remain comparable, researchers can distinguish a general growth capacity from a condition-specific response. This pattern-based comparison is especially useful when screening many colonies for distinct phenotypes in a single experiment.
Researchers first establish colonies on a master agar plate, then press a sterile velvet or similar surface against it so cells from each colony are collected in their original arrangement. The surface is pressed onto one or more fresh plates containing selected media or conditions. After growth, corresponding positions are compared across the plates.
The essential materials are a master agar plate, one or more fresh agar plates, and a sterile velvet or similar transfer surface. The fresh plates must provide the differing media or conditions being tested, while the master plate supplies the original colony pattern. Preserving the relative positions during transfer makes later comparisons meaningful.
Researchers use the technique when they need to screen many microbial colonies for condition-dependent traits while retaining their relationship to an original population. Supported applications include bacterial genetics, microbial physiology, selection experiments, and mutation screening. It can help locate auxotrophic mutants, antibiotic-resistant cells, or organisms affected by particular nutrients and environmental conditions.
The resulting growth patterns can identify colonies with contrasting nutritional requirements, antibiotic responses, or sensitivity to environmental conditions. These observations support the detection of auxotrophic mutants and antibiotic-resistant cells, while also informing studies of microbial physiology and selection. Because each outcome remains tied to a master-plate position, researchers can relate the phenotype to its source colony.