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Biochemistry

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Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Article DOI: 10.3791/54488-v • 10:39 min • November 28th, 2016
November 28th, 2016 •
*1, *1, 1, 2, 1, 3, 1,2
1Department of Medicine, Vanderbilt University School of Medicine, 2Center for Quantitative Sciences, Vanderbilt University School of Medicine, 3Department of Cancer Biology, Vanderbilt University School of Medicine
* These authors contributed equally

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This protocol describes the isolation and quantification of high-density lipoprotein small RNAs.

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Isolation High-density Lipoproteins Non-coding Small RNA Quantification Protocol Lipoprotein Field Small RNA Signatures Health Disease Biomarkers Cell To Cell Messages Technique Pure Lipoproteins Small RNA Changes Cardiometabolic Diseases Extracellular RNAs Drug Targets HDL Small RNAs Low Density Lipoprotein Small RNAs Equipment Expertise Samples Preparation Density-gradient Ultracentrifugation Established Methods High Throughput Sequencing Real-time PCR
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