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Biochemistry

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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Article DOI: 10.3791/54535-v • 07:46 min • October 1st, 2016
October 1st, 2016 •
1, 1, 1, 1
1Division of Analytical Biosciences, Leiden Academic Center for Drug Research, Leiden University

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A protocol for metabolic profiling of biological samples by capillary electrophoresis–mass spectrometry using a sheathless porous tip interface design is presented.

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Sheathless Capillary Electrophoresis-mass Spectrometry Metabolic Profiling Biological Samples Highly Polar Metabolites Charged Metabolites Electrolitic Separations Sensitivity Interfacing Robustness Sheath As Free Interface Profiling Clinical Questions Metabolomics Charge To Size Ratio Anionic Metabolites Cationic Metabolites CE Voltage Polarity MS Detection Polarity
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