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Journal / Neuroscience / Immunfärgning av biocytin fyllda och bearbetade…
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Neuroscience

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JoVE Journal Neuroscience
Immunfärgning av biocytin fyllda och bearbetade Sektioner neurokemiska markörer
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Immunfärgning av biocytin fyllda och bearbetade Sektioner neurokemiska markörer

Article DOI: 10.3791/54880-v • 08:54 min • December 31st, 2016
December 31st, 2016 •
1, 2, 2, 2
1Graduate School of Biomedical Sciences, Rutgers New Jersey Medical School, 2Department of Pharmacology, Physiology and Neuroscience, Rutgers New Jersey Medical School

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Detta protokoll presenterar en metod för den morfologiska återvinning av nervceller lappade under elektrofysiologiska inspelningar med biocytin fyllning och efterföljande immunhistokemisk efterbearbetning. Vi visar att tjocka biocytin fyllda sektioner som färgats och täck kan restained med andra primära dagar eller månader antikropps senare.

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Neurovetenskap morfologi biocytin immunohistokemi neurokemiska markör dubbel märkning patch clamp
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