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Immunology and Infection

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JoVE Journal Immunology and Infection
Intracellulær Farvning og flowcytometri at identificere lymfocytundergrupper inden Murine Aorta, Nyre og lymfeknuder i en model af hypertension
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Intracellulær Farvning og flowcytometri at identificere lymfocytundergrupper inden Murine Aorta, Nyre og lymfeknuder i en model af hypertension

Article DOI: 10.3791/55266-v • 09:20 min • January 28th, 2017
January 28th, 2017 •
1, 2, 3, 1
1Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University

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Denne artikel giver detaljeret metode til at identificere og kvantificere funktionelle T lymfocyt delmængder stede i murine nyre, aorta og lymfeknuder ved intracellulær farvning og flowcytometri. Modellen af ​​angiotensin II-induceret hypertension blev valgt til at forklare, trin-for-trin, de procedurer og grundlæggende principper for flowcytometri og intracellulær farvning.

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Immunologi intracellulær farvning flowcytometri T-celler lymfocytundergrupper cytokiner hypertension
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