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Neuroscience

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JoVE Journal Neuroscience
Modellierung von neuronalen Tod und Degeneration in Maus primären zerebelläre Granulat Neuronen
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Modellierung von neuronalen Tod und Degeneration in Maus primären zerebelläre Granulat Neuronen

Article DOI: 10.3791/55871-v • 10:36 min • November 6th, 2017
November 6th, 2017 •
1,2, 1, 3, 1,2,4
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Integrated Program in Neuroscience, McGill University, 3Department of Cellular and Molecular Medicine, University of Ottawa, 4Department of Oncology, Faculty of Medicine, McGill University

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Dieses Protokoll beschreibt eine einfache Methode zur Isolierung und Kultivierung primäre Maustaste zerebralen Granulat Neuronen (CGNs) von 6-7 Tage alten Welpen, effizienten Transduktion von CGNs für Verlust und Gewinn von Funktionsstudien, und Modellierung NMDA-induzierten neuronalen Excitotoxizität, niedrig-Kalium-induzierte Zelltod, DNA-Schäden und oxidativen Stress mit der gleichen Kultur-Modell.

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Neurowissenschaften Ausgabe 129 Kleinhirn zerebelläre Granulat Neuronen (CGN) Adenovirus Lentivirale Transduktion Excitotoxizität N-Methyl-D-Aspartat-(NMDA) Apoptose Kaliumchlorid (KCl) Camptothecin Wasserstoffperoxid (H2O2)
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