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En kometanalysetilnærming med høy gjennomstrømning for å vurdere cellulær DNA-skade
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En kometanalysetilnærming med høy gjennomstrømning for å vurdere cellulær DNA-skade

Article DOI: 10.3791/63559-v • 07:57 min • May 10th, 2022
May 10th, 2022 •
*1, *2, *3, 4, 5, 1
1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences, University of South Florida, 2Cepheid (Danaher Corp.), US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment, Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University
* These authors contributed equally

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Kometanalysen er et populært middel for å oppdage DNA-skade. Denne studien beskriver en tilnærming til å kjøre lysbilder i representative varianter av kometanalysen. Denne tilnærmingen økte antall prøver betydelig samtidig som den reduserte analysens kjøretid, antall lysbildemanipulasjoner og risikoen for skade på geler.

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Biologi utgave 183 Kometanalyse DNA-skade DNA-reparasjon oksidativt stress biomarkør elektroforese
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