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Un enfoque de ensayo cometario de alto rendimiento para evaluar el daño del ADN celular
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Un enfoque de ensayo cometario de alto rendimiento para evaluar el daño del ADN celular

Article DOI: 10.3791/63559-v • 07:57 min • May 10th, 2022
May 10th, 2022 •
*1, *2, *3, 4, 5, 1
1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences, University of South Florida, 2Cepheid (Danaher Corp.), US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment, Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University
* These authors contributed equally

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El ensayo del cometa es un medio popular para detectar daños en el ADN. Este estudio describe un enfoque para ejecutar diapositivas en variantes representativas del ensayo de cometas. Este enfoque aumentó significativamente el número de muestras al tiempo que disminuyó el tiempo de ejecución del ensayo, el número de manipulaciones de portaobjetos y el riesgo de daño a los geles.

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Biología Número 183 Ensayo cometa Daño en el ADN reparación del ADN estrés oxidativo biomarcador electroforesis
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