Analisi della morfologia organoide rettale (ROMA): un saggio diagnostico nella fibrosi cistica

2.8K visualizzazioni

07:56 min.

10 giugno 2022

2.8K visualizzazioni

Senne Cuyx¹^,² , Anabela S. Ramalho¹ , Nikky Corthout³^,⁴ , Steffen Fieuws⁵ , Eva Fürstová⁶ , Kaline Arnauts⁷^,⁸ , Marc Ferrante⁷^,⁹ , Catherine Verfaillie⁸ , Sebastian Munck³^,⁴ , Mieke Boon¹^,² , Marijke Proesmans¹^,² , Lieven Dupont¹⁰^,¹¹ , Kris De Boeck¹^,² , François Vermeulen¹^,²

¹Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, ²Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, ³VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, ⁴Department for Neuroscience, KU Leuven, ⁵Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, ⁶Department of Pediatrics, 2<sup>nd</sup> Faculty of Medicine, Charles University and Motol University Hospital, ⁷Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, ⁸Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, ⁹Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, ¹⁰Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, ¹¹Department of Respiratory Diseases, University Hospitals Leuven

Questo protocollo descrive l'analisi morfologica degli organoidi rettali (ROMA), un nuovo test diagnostico per la fibrosi cistica (FC). Le caratteristiche morfologiche, vale a dire la rotondità (indice di circolarità, CI) e la presenza di un lume (rapporto di intensità, IR), sono una misura della funzione CFTR. L'analisi di 189 soggetti ha mostrato una perfetta discriminazione tra FC e non CF.