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Medición del número de copias de ADN mitocondrial unicelular y la heteroplasmia mediante la reacción en cadena de la polimerasa de gotitas digitales

Article DOI: 10.3791/63870-v • 09:15 min • July 12th, 2022
July 12th, 2022 •
1,2, 1,2
1Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus, 2Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus

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Aquí presentamos un protocolo para medir el número absoluto de copias de ADN mitocondrial (mt) y los niveles de heteroplasmia de deleción de ADNmt en células individuales.

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Medicina Número 185
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