Generazione di colture di interfaccia aria-liquido di cellule epiteliali delle vie aeree da cellule staminali pluripotenti umane
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June 14, 2022
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I recenti progressi nei protocolli di differenziazione delle cellule staminali pluripotenti indotte dall'uomo consentono la derivazione graduale di tipi di cellule specifiche per organo. Qui, forniamo passaggi dettagliati per il mantenimento e l'espansione delle cellule basali delle vie aeree derivate da iPSC e la loro differenziazione in un epitelio mucociliare in colture di interfaccia aria-liquido.
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Cite this Article
Berical, A., Beermann, M. L., Suzuki, S., LeSuer, J., Matte, T., Davis, B., Kotton, D., Hawkins, F. Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells. J. Vis. Exp. (184), e63882, doi:10.3791/63882 (2022).
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