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Neuroscience

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JoVE Journal Neuroscience
Robust vævsfabrikation til langsigtet kultur af iPSC-afledte hjerneorganoider til aldringsforskning
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Robust vævsfabrikation til langsigtet kultur af iPSC-afledte hjerneorganoider til aldringsforskning

Article DOI: 10.3791/64586-v • 08:20 min • May 12th, 2023
May 12th, 2023 •
1, 2,3, 1
1Department of Applied Stem Cell Technologies, TechMed Centre Enschede, University of Twente, 2Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, 3Department of Genetics, Harvard Medical School

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Denne protokol indeholder en trinvis procedure for reproducerbar generering, vedligeholdelse og ældning af cerebrale organoider afledt af menneskeskabte pluripotente stamceller (iPSC'er). Denne metode muliggør dyrkning og modning af cerebrale organoider i længere perioder, hvilket letter modellering af processer involveret i hjernens aldring og aldersrelateret patogenese.

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Neurovidenskab nummer 195
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