Ipsc-derived Organoids
Ipsc-derived Organoids - Related Videos
07:40
Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
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Cited by 46 •
2017
Modeling human brain development has been hindered due to the unprecedented complexity of neural epithelial tissue. Here, a method for the robust generation of brain organoids to delineate early events of human brain development and to model microcephaly in vitro is described.
05:23
Developing Human iPSC-Derived Motor Nerve Organoids Using a Microfluidic Chip
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2025
This video demonstrates the method of creating motor nerve organoids by first culturing human induced pluripotent stem cells in a special medium to form spheroids, then differentiating them into motor neurons using specific inhibitors, and finally cultivating them in a microfluidic chip to extend axons and form motor nerve organoids.
05:06
Three-Dimensional Thymic Culture System to Generate iPSC-Derived Thymic Emigrants
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2025
This video demonstrates a protocol for generating functional antigen-specific T cells from iPSC-derived immature T cells using a three-dimensional thymic culture system. Co-culturing the iPSC-derived immature T cells with endogenous lymphocyte-depleted mice fetal thymic lobes resulted in successful maturation of the T cells to form CD8αβ+ MHC class I+ iPSC-derived thymic emigrants (iTEs).
07:53
Generating a Blood-Brain Barrier Chip Using iPSC-derived Precursor Cells
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2025
This video showcases creating a blood-brain barrier chip using iPSC-derived precursor cells. It involves seeding neural progenitor cells and brain microvascular endothelial cells in separate channels with a porous membrane. By flowing differentiation media, the endothelial cells form tight junctions resembling blood vessels, and the neural cells mature into various brain cell types, establishing a functional blood-brain barrier on the chip.
05:44
Generating Induced Pluripotent Stem Cell-Derived Cerebral Organoids
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2025
This video demonstrates an assay to generate cerebral organoids from induced pluripotent stem cells (iPSCs). Human iPSCs are induced to form embryoid bodies (EBs). The EBs are cultured over a polymer scaffold using an induction medium, promoting differentiation into neural stem cells, progenitor cells, and immature neurons. Finally, the EBs are embedded in a matrix and transferred to a maintenance medium for cerebral organoid formation.
FAQs
Reprogramming returns mature cells to a pluripotent state, giving them the developmental potential needed for subsequent neural specification. Researchers then use defined culture conditions to guide differentiation toward neural lineages. Once those cells begin differentiating, self-organization contributes to the formation of tissue-like architectures, allowing developmental processes and neuronal maturation to emerge in a laboratory model.
Three-dimensional organization provides tissue-like architecture that conventional two-dimensional cultures do not fully reproduce. This structure helps researchers examine aspects of brain development, neuronal maturation, and disease-related changes within a more complex cellular arrangement. The added organization can therefore reveal features of neural biology that may be difficult to study in simpler cell culture systems.
Defined culture conditions direct pluripotent cells toward selected neural lineages and influence how differentiation proceeds. They are therefore central to controlling the developmental trajectory of the culture rather than serving only as a maintenance environment. In neuroscience experiments, this guidance supports the generation of structures suitable for examining brain development, maturation, and disease-associated changes.
The workflow starts by reprogramming mature cells into induced pluripotent stem cells. Researchers then apply defined culture conditions to guide those cells toward neural lineages, followed by continued culture that permits differentiation and self-organization. These processes produce increasingly complex tissue-like architectures that can be examined for developmental features, neuronal maturation, or disease-related changes.
The process depends on an appropriate starting population of reprogrammed pluripotent cells and on defined culture conditions that promote neural differentiation. Continued culture is also important because cell differentiation and self-organization contribute to architectural development. Together, these inputs determine whether the resulting structures are suitable for investigating the intended neural or disease-related question.
Researchers use these models when they need to investigate human brain development, neuronal maturation, or disease-related changes in a laboratory setting. They are particularly relevant to studies of neurodevelopmental disorders, where conventional cell cultures may not capture sufficient tissue complexity. The models can also support evaluations of therapeutic responses and analyses of patient-specific biology.
Because mature cells can be reprogrammed and then directed toward neural lineages, the resulting cultures can support investigations of patient-specific biology. Researchers can examine disease-related changes in a human-derived neural model and study how those structures respond to potential therapies. This makes the approach relevant to neurodevelopmental disorder research and therapeutic-response studies.