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JoVE Journal Neuroscience
Robust vevsfabrikasjon for langsiktig kultur av iPSC-avledede hjerneorganoider for aldringsforskning
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Robust vevsfabrikasjon for langsiktig kultur av iPSC-avledede hjerneorganoider for aldringsforskning

Article DOI: 10.3791/64586-v • 08:20 min • May 12th, 2023
May 12th, 2023 •
1, 2,3, 1
1Department of Applied Stem Cell Technologies, TechMed Centre Enschede, University of Twente, 2Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, 3Department of Genetics, Harvard Medical School

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Den nåværende protokollen gir en trinnvis prosedyre for reproduserbar generering, vedlikehold og aldring av cerebrale organoider avledet fra humaninduserte pluripotente stamceller (iPSCs). Denne metoden muliggjør dyrking og modning av cerebrale organoider i lengre perioder, noe som letter modelleringen av prosesser involvert i hjernens aldring og aldersrelatert patogenese.

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