Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Andre Lai; Rachel Rex; Kristen L. Cotner; Alan Dong; Michael Lustig; Lydia L. Sohn

doi:10.3791/64665

Rilevamento meccano-nodo-pore: una piattaforma rapida e senza etichette per misure viscoelastiche...

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Bioengineering

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JoVE Journal Bioengineering

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Rilevamento meccano-nodo-pore: una piattaforma rapida e senza etichette per misure viscoelastiche monocellulari multiparametriche

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05:49 min

December 2, 2022

DOI: 10.3791/64665-v

Andre Lai*¹, Rachel Rex*², Kristen L. Cotner¹, Alan Dong³, Michael Lustig^1,3, Lydia L. Sohn^1,2

¹Graduate Program in Bioengineering,University of California, Berkeley and University of California, San Francisco, ²Department of Mechanical Engineering,University of California, Berkeley, ³Department of Electrical Engineering and Computer Sciences,University of California, Berkeley

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Overview

This article presents a method for mechanically phenotyping single cells using an electronics-based microfluidic platform known as mechano-node-pore sensing (mechano-NPS). The platform enables the measurement of both elastic and viscous properties of cells at a throughput of up to 10 cells per second, facilitating minimal sample preparation and non-destructive analysis.

Key Study Components

Area of Science

Neuroscience
Cell Biology
Microfluidics

Background

Mechano-NPS is an innovative platform for studying single cell mechanics.
The method allows for the analysis of various cell types, including primary samples.
It provides insights into the viscoelastic properties of cells.
The approach is compatible with downstream analyses, enhancing its utility in research.

Purpose of Study

To develop a method for mechanical phenotyping of single cells.
To measure the elastic and viscous properties of cells efficiently.
To facilitate understanding of cell behavior and disease progression.

Methods Used

Electronics-based microfluidic platform for cell analysis.
Measurement of single cell viscoelasticity.
Minimal sample preparation required.
Non-destructive measurement compatible with further analyses.

Main Results

Successful measurement of elastic and viscous properties of various cell types.
Demonstrated application of mechano-NPS in studying subcellular components.
Potential for monitoring drug responses and understanding disease mechanisms.

Conclusions

Mechano-NPS is a valuable tool for mechanical phenotyping of cells.
The platform offers a cost-effective alternative to traditional methods.
It opens new avenues for research in cell mechanics and biophysics.

Frequently Asked Questions

What is mechano-node-pore sensing?

Mechano-node-pore sensing is an electronics-based microfluidic platform used for mechanical phenotyping of single cells.

How does mechano-NPS compare to traditional methods?

Mechano-NPS is more cost-effective and requires minimal sample preparation compared to traditional optical methods.

What types of cells can be analyzed using this method?

The method can be applied to various cell types, including primary samples.

What properties of cells are measured?

The platform measures both elastic and viscous properties of single cells.

Is the measurement process destructive?

No, the mechano-NPS method is non-destructive and compatible with downstream analyses.

What are the potential applications of this technology?

It can be used to understand cell behavior, monitor drug responses, and determine disease progression.

Presentato qui è un metodo per fenotipizzare meccanicamente singole cellule utilizzando una piattaforma microfluidica basata sull'elettronica chiamata mechano-node-pore sensing (mechano-NPS). Questa piattaforma mantiene un throughput moderato di 1-10 cellule / s misurando sia le proprietà biofisiche elastiche che viscose delle cellule.

Presentiamo una piattaforma microfluidica basata sull'elettronica per la fenotipizzazione meccanica di singole cellule. In particolare, misuriamo le proprietà elastiche e viscose di una singola cella con una produttività fino a 10 celle al secondo. Il nostro metodo richiede una preparazione minima del campione, utilizza una semplice misurazione elettronica, sostituendo il costoso hardware ottico e l'analisi delle immagini complesse, e non è distruttivo, il che significa che il nostro approccio è compatibile con le analisi a valle.

Mechano-NPS è stato applicato a molti tipi di cellule, compresi i campioni primari, e ha misurato l'effetto dei componenti subcellulari sulla viscoelasticità delle singole cellule. Potrebbe essere usato per comprendere il comportamento cellulare, determinare la progressione della malattia o monitorare la risposta ai farmaci. Per iniziare, rimuovere i componenti trattati al plasma dalla camera al plasma.

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