Name: Effetto estrogeno-simile della capsula di Bazi Bushen nei ratti ovariectomizzati (Video) | JoVE Journal
Uploaded: 2023-04-07T14:40:00.000+00:00
Duration: 8 min 56 s
Description: Esplora gli effetti estrogeno-simili della capsula Bazi Bushen sulla salute ossea in ratti ovariectomizzati. Questo protocollo utilizza micro-CT e analisi istologiche per valutare il metabolismo osseo e la via di segnalazione RANKL/OPG.

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<table><tbody><tr><td>10% formalina tamponata neutra</td><td>Proteintech Group, Inc.</td><td>23408-1AP-100</td><td></td></tr><tr><td>Kit ELISA 17-beta-estradiolo</td><td>Proteintech Group, Inc.</td><td>21933-1-AP</td><td></td></tr><tr><td>3%H2O2</td><td>ShanDong LIRCON Medical Technology Incorporated Company</td><td>20221027</td><td></td></tr><tr><td>Anticorpo anti-osteoprotegerina</td><td>Abcam</td><td>ab203061</td><td></td></tr><tr><td>Capsule di Bazibushen</td><td>Shijiazhuang Yiling Pharmaceutical Co., Ltd.</td><td></td><td></td>XB2103001</tr><tr><td>Biotina capra anti-coniglio IgG</td><td>Abcam</td><td>ab207995</td><td></td></tr><tr><td>Kit per il dosaggio del calcio</td><td>Nanjing Jiancheng Bioengineering Institute</td><td>C004-2-1</td><td></td></tr><tr><td>Diaminobenzidina</td><td>ZSGB-BIO</td><td>ZLI-9018</td><td></td></tr><tr><td>Compresse di estradiolo valerato</td><td>Bayer AG</td><td>156A</td><td></td></tr><tr><td>Gene 1580R centrifuga</td><td>GENE Co. Ltd.</td><td></td><td></td>GZ422515090077</tr><tr><td>Image-Pro Plus</td><td>Media Cybernetics</td><td>IPP 6.0</td><td></td></tr><tr><td>Leica DM6000B Microscope (sistema di microscopio verticale completamente automatizzato)</td><td>Leica</td><td>361715</td><td></td></tr><tr><td>Normal Goat Serum Blocking Solution</td><td>Vector Laboratories</td><td>S-1000-20</td><td></td></tr><tr><td>Kit per il dosaggio del fosfato</td><td>Nanjing Jiancheng Bioengineering Institute</td><td>C006-1-1</td><td></td></tr><tr><td>Soluzione salina tamponata con fosfato</td><td>Servicebio, Wuhan, Cina</td><td>CR10201M</td><td></td></tr><tr><td>Sistema di imaging microCT Quantum GX2</td><td>PerkinElmer</td><td>CLS149276</td><td></td></tr><tr><td>RANKL Anticorpo policlonale di coniglio</td><td>Elabscience Biotechnology Co. Ltd.</td><td>E-EL-R3032-96T</td><td></td></tr><tr><td>Kit ELISA SOST per ratto (sclerostina)</td><td>Elabscience Biotechnology Co., Ltd.</td><td>E-EL-R1405c-96T</td><td></td></tr><tr><td>Rat TRACP-5b (fosfatasi acida resistente al tartrato 5b) kit ELISA</td><td>Abcam</td><td>ab108667</td><td></td></tr><tr><td>Rat &beta;-CTx (Beta Crosslaps) kit ELISA</td><td>Elabscience Biotechnology Co., Ltd.</td><td>E-EL-R0939c-96T</td><td></td></tr><tr><td>Anticorpo policlonale di coniglio sclerostina</td><td>Beijing Vital River Laboratory Animal Technology Co., Ltd.</td><td>SCXK(JING)2016-0006</td><td></td></tr><tr><td>Scanner per vetrini</td><td>Hamamatsu Photonics K.K.</td><td>Nano Zoomer-SQ</td><td></td></tr><tr><td>Sprague Dawley ratti femmina</td><td>Pechino Vital River Laboratory Animal Technology Co., Ltd.</td><td>ZLI-9381</td><td></td></tr></tbody></table>

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Effetto estrogeno-simile della capsula di Bazi Bushen nei ratti ovariectomizzati

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