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Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

Article DOI: 10.3791/64886-v • 05:22 min • February 24th, 2023
February 24th, 2023 •
1, 2,3, 2, 1,2, 1,2, 4,5, 2,3, 2,3, 1,2
1Division of Oncology, Children’s Hospital of Philadelphia, 2Center for Data Driven Discovery in Biomedicine, Children’s Hospital of Philadelphia, 3Division of Neurosurgery, Children’s Hospital of Philadelphia, 4Ben Towne Center for Childhood Cancer Research, Seattle Children’s Research Institute, 5Department of Pediatrics, Seattle Children’s Hospital, University of Washington

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Central nervous system (CNS) tumors are the leading cause of cancer-related death in children, and locoregional immune-based therapies are increasingly being tested for patients in clinical trials. This protocol describes methods for locoregional cannula implantation in mice for the preclinical evaluation of immunotherapeutic infusions targeting CNS tumors.

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Intracranial Cannula Implantation Serial Locoregional CAR T-cell Infusions Mice Intraventricular Catheter System Local Regional Delivery CAR T-cell Therapy Central Nervous System Tumors Invasive Surgical Procedures Therapeutics Neuroscience Stereotactic Arm Cannula Bite Bar Inhalation Anesthesia Isoflurane Stereotaxic Apparatus Surgical Site Skull Landmarks Bregma Lambda
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