Name: Sviluppo di un modello di inalazione preclinica per testare i distillati di cannabis vaporizzati (Video) | JoVE Journal
Uploaded: 2025-05-30T13:55:00.000+00:00
Duration: 6 min 9 s
Description: Scopri come sviluppare un modello murino di inalazione per studiare gli effetti dei distillati di cannabis vaporizzati sulla funzione polmonare. Questo metodo consente di valutare le alterazioni trascrizionali nelle cellule epiteliali e i livelli di THC-COOH nel siero.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Spindle, T. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+Effects+of+Smoked+and+Vaporized+Cannabis+in+Healthy+Adults+Who+Infrequently+Use+Cannabis%3A+A+Crossover+Trial%5BTitle%5D)+AND+%22JAMA+Netw+Open%22%5BJournal%5D)">Acute Effects of Smoked and Vaporized Cannabis in Healthy Adults Who Infrequently Use Cannabis: A Crossover Trial.</a> JAMA Netw Open. 1 (7), e184841-e184841 (2018).</li>
<li>Morean, M. E., Kong, G., Camenga, D. R., Cavallo, D. A., Krishnan-Sarin, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High+School+Students%27+Use+of+Electronic+Cigarettes+to+Vaporize+Cannabis%5BTitle%5D)+AND+%22Pediatrics%22%5BJournal%5D)">High School Students' Use of Electronic Cigarettes to Vaporize Cannabis.</a> Pediatrics. 136 (4), 611-616 (2015).</li>
<li>Russell, C., Rueda, S., Room, R., Tyndall, M., Fischer, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Routes+of+administration+for+cannabis+use+-+basic+prevalence+and+related+health+outcomes%3A+A+scoping+review+and+synthesis%5BTitle%5D)+AND+%22Int+J+Drug+Policy%22%5BJournal%5D)">Routes of administration for cannabis use - basic prevalence and related health outcomes: A scoping review and synthesis.</a> Int J Drug Policy. 52, 87-96 (2018).</li>
<li>Lazarjani, M. P., Young, O., Kebede, L., Seyfoddin, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Processing+and+extraction+methods+of+medicinal+cannabis%3A+a+narrative+review%5BTitle%5D)+AND+%22J+Cannabis+Res%22%5BJournal%5D)">Processing and extraction methods of medicinal cannabis: a narrative review.</a> J Cannabis Res. 3 (1), 32(2021).</li>
<li>Jones, C. B., Hill, M. L., Pardini, D. A., Meier, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Prevalence+and+correlates+of+vaping+cannabis+in+a+sample+of+young+adults%5BTitle%5D)+AND+%22Psychol+Addict+Behav%22%5BJournal%5D)">Prevalence and correlates of vaping cannabis in a sample of young adults.</a> Psychol Addict Behav. 30 (8), 915-921 (2016).</li>
<li>2023–24 Departmental Plan: Health Canada. , 
 <a target="_blank" href="https://www.canada.ca/en/health-canada/corporate/transparency/corporate-management-reporting/report-plans-priorities/2023-2024-departmental-plan.html">https://www.canada.ca/en/health-canada/corporate/transparency/corporate-management-reporting/report-plans-priorities/2023-2024-departmental-plan.html</a> (2023).</li>
<li>Geweda, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+dispensaries%27+cannabis+flowers+for+accuracy+of+labeling+of+cannabinoids+content%5BTitle%5D)+AND+%22J+Cannabis+Res%22%5BJournal%5D)">Evaluation of dispensaries' cannabis flowers for accuracy of labeling of cannabinoids content.</a> J Cannabis Res. 6 (1), 11(2024).</li>
<li>Meehan-Atrash, J., Rahman, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabis+Vaping%3A+Existing+and+Emerging+Modalities%2C+Chemistry%2C+and+Pulmonary+Toxicology%5BTitle%5D)+AND+%22Chem+Res+Toxicol%22%5BJournal%5D)">Cannabis Vaping: Existing and Emerging Modalities, Chemistry, and Pulmonary Toxicology.</a> Chem Res Toxicol. 34 (10), 2169-2179 (2021).</li>
<li>Van der Kooy, F., Pomahacova, B., Verpoorte, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabis+smoke+condensate+I%3A+the+effect+of+different+preparation+methods+on+tetrahydrocannabinol+levels%5BTitle%5D)+AND+%22Inhal+Toxicol%22%5BJournal%5D)">Cannabis smoke condensate I: the effect of different preparation methods on tetrahydrocannabinol levels.</a> Inhal Toxicol. 20 (9), 801-804 (2008).</li>
<li>Rosenberg, E. C., Patra, P. H., Whalley, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Therapeutic+effects+of+cannabinoids+in+animal+models+of+seizures%2C+epilepsy%2C+epileptogenesis%2C+and+epilepsy-related+neuroprotection%5BTitle%5D)+AND+%22Epilepsy+Behav%22%5BJournal%5D)">Therapeutic effects of cannabinoids in animal models of seizures, epilepsy, epileptogenesis, and epilepsy-related neuroprotection.</a> Epilepsy Behav. 70 (Pt B), 319-327 (2017).</li>
<li>National Academies of Sciences, Engineering, and Medicine; Health and Medicine Division. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22The+Health+Effects+of+Cannabis+and+Cannabinoids%3A+The+Current+State+of+Evidence+and+Recommendations+for+Research%22">The Health Effects of Cannabis and Cannabinoids: The Current State of Evidence and Recommendations for Research</a>. , (2017).</li>
<li>Been, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+low-level+JUUL+aerosol+exposure+causes+pulmonary+immunologic%2C+transcriptomic%2C+and+proteomic+changes%5BTitle%5D)+AND+%22Faseb+J%22%5BJournal%5D)">Chronic low-level JUUL aerosol exposure causes pulmonary immunologic, transcriptomic, and proteomic changes.</a> Faseb J. 37 (2), e22732(2023).</li>
<li>Been, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+impact+of+JUUL+flavors+on+pulmonary+immune+modulation+and+oxidative+stress+responses+in+male+and+female+mice%5BTitle%5D)+AND+%22Arch+Toxicol%22%5BJournal%5D)">Differential impact of JUUL flavors on pulmonary immune modulation and oxidative stress responses in male and female mice.</a> Arch Toxicol. 96 (6), 1783-1798 (2022).</li>
<li>Caruana, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+exposure+to+E-cigarette+aerosols+potentiates+atherosclerosis+in+a+sex-dependent+manner%5BTitle%5D)+AND+%22Toxicol+Appl+Pharmacol%22%5BJournal%5D)">Chronic exposure to E-cigarette aerosols potentiates atherosclerosis in a sex-dependent manner.</a> Toxicol Appl Pharmacol. 492, 117095(2024).</li>
<li>Paoli, S., Eidelman, D. H., Mann, K. K., Baglole, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sex-specific+alterations+in+pulmonary+metabolic%2C+xenobiotic+and+lipid+signalling+pathways+after+e-cigarette+aerosol+exposure+during+adolescence+in+mice%5BTitle%5D)+AND+%22BMJ+Open+Respir+Res%22%5BJournal%5D)">Sex-specific alterations in pulmonary metabolic, xenobiotic and lipid signalling pathways after e-cigarette aerosol exposure during adolescence in mice.</a> BMJ Open Respir Res. 11 (1), e002423(2024).</li>
<li>Lamb, T., Muthumalage, T., Meehan-Atrash, J., Rahman, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nose-Only+Exposure+to+Cherry-+and+Tobacco-Flavored+E-Cigarettes+Induced+Lung+Inflammation+in+Mice+in+a+Sex-Dependent+Manner%5BTitle%5D)+AND+%22Toxics%22%5BJournal%5D)">Nose-Only Exposure to Cherry- and Tobacco-Flavored E-Cigarettes Induced Lung Inflammation in Mice in a Sex-Dependent Manner.</a> Toxics. 10 (8), 471(2022).</li>
<li>Wang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protein+thiol+oxidation+in+the+rat+lung+following+e-cigarette+exposure%5BTitle%5D)+AND+%22Redox+Biol%22%5BJournal%5D)">Protein thiol oxidation in the rat lung following e-cigarette exposure.</a> Redox Biol. 37, 101758(2020).</li>
<li>Gazarov, E. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pharmacokinetics+of+delta-9-tetrahydrocannabinol+following+acute+cannabis+smoke+exposure+in+mice%3B+effects+of+sex%2C+age%2C+and+strain%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">Pharmacokinetics of delta-9-tetrahydrocannabinol following acute cannabis smoke exposure in mice; effects of sex, age, and strain.</a> Front Pharmacol. 14, 1227220(2023).</li>
<li>Haidar, Z., Traboulsi, H., Eidelman, D. H., Baglole, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+inflammatory+profile+in+the+lungs+of+mice+exposed+to+cannabis+smoke+with+varying+THC%3ACBD+ratio%5BTitle%5D)+AND+%22Arch+Toxicol%22%5BJournal%5D)">Differential inflammatory profile in the lungs of mice exposed to cannabis smoke with varying THC:CBD ratio.</a> Arch Toxicol. 97 (7), 1963-1978 (2023).</li>
<li>Behar, R. Z., Hua, M., Talbot, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Puffing+topography+and+nicotine+intake+of+electronic+cigarette+users%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Puffing topography and nicotine intake of electronic cigarette users.</a> PLoS One. 10 (2), e0117222(2015).</li>
<li>Leavens, E. L. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((electronic+cigarette+use+patterns%2C+other+tobacco+product+use%2C+and+reasons+for+use+among+ever+users%3A+Results+from+a+convenience+sample%5BTitle%5D)+AND+%22Addict+Behav%22%5BJournal%5D)">electronic cigarette use patterns, other tobacco product use, and reasons for use among ever users: Results from a convenience sample.</a> Addict Behav. 95, 178-183 (2019).</li>
<li>Huestis, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+cannabinoid+pharmacokinetics%5BTitle%5D)+AND+%22Chem+Biodivers%22%5BJournal%5D)">Human cannabinoid pharmacokinetics.</a> Chem Biodivers. 4 (8), 1770-1804 (2007).</li>
<li>Hložek, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pharmacokinetic+and+behavioural+profile+of+THC%2C+CBD%2C+and+THC%2BCBD+combination+after+pulmonary%2C+oral%2C+and+subcutaneous+administration+in+rats+and+confirmation+of+conversion+in+vivo+of+CBD+to+THC%5BTitle%5D)+AND+%22Eur+Neuropsychopharmacol%22%5BJournal%5D)">Pharmacokinetic and behavioural profile of THC, CBD, and THC+CBD combination after pulmonary, oral, and subcutaneous administration in rats and confirmation of conversion in vivo of CBD to THC.</a> Eur Neuropsychopharmacol. 27 (12), 1223-1237 (2017).</li>
<li>Nasrin, S., Watson, C. J. W., Perez-Paramo, Y. X., Lazarus, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabinoid+Metabolites+as+Inhibitors+of+Major+Hepatic+CYP450+Enzymes%2C+with+Implications+for+Cannabis-Drug+Interactions%5BTitle%5D)+AND+%22Drug+Metab+Dispos%22%5BJournal%5D)">Cannabinoid Metabolites as Inhibitors of Major Hepatic CYP450 Enzymes, with Implications for Cannabis-Drug Interactions.</a> Drug Metab Dispos. 49 (12), 1070-1080 (2021).</li>
<li>Hartman, R. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Controlled+Cannabis+Vaporizer+Administration%3A+Blood+and+Plasma+Cannabinoids+with+and+without+Alcohol%5BTitle%5D)+AND+%22Clin+Chem%22%5BJournal%5D)">Controlled Cannabis Vaporizer Administration: Blood and Plasma Cannabinoids with and without Alcohol.</a> Clin Chem. 61 (6), 850-869 (2015).</li>
<li>Newmeyer, M. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Free+and+Glucuronide+Whole+Blood+Cannabinoids%27+Pharmacokinetics+after+Controlled+Smoked%2C+Vaporized%2C+and+Oral+Cannabis+Administration+in+Frequent+and+Occasional+Cannabis+Users%3A+Identification+of+Recent+Cannabis+Intake%5BTitle%5D)+AND+%22Clin+Chem%22%5BJournal%5D)">Free and Glucuronide Whole Blood Cannabinoids' Pharmacokinetics after Controlled Smoked, Vaporized, and Oral Cannabis Administration in Frequent and Occasional Cannabis Users: Identification of Recent Cannabis Intake.</a> Clin Chem. 62 (12), 1579-1592 (2016).</li>
<li>Schwope, D. M., Karschner, E. L., Gorelick, D. A., Huestis, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Identification+of+recent+cannabis+use%3A+whole-blood+and+plasma+free+and+glucuronidated+cannabinoid+pharmacokinetics+following+controlled+smoked+cannabis+administration%5BTitle%5D)+AND+%22Clin+Chem%22%5BJournal%5D)">Identification of recent cannabis use: whole-blood and plasma free and glucuronidated cannabinoid pharmacokinetics following controlled smoked cannabis administration.</a> Clin Chem. 57 (10), 1406-1414 (2011).</li>
<li>Sharma, P., Murthy, P., Bharath, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chemistry%2C+metabolism%2C+and+toxicology+of+cannabis%3A+clinical+implications%5BTitle%5D)+AND+%22Iran+J+Psychiatry%22%5BJournal%5D)">Chemistry, metabolism, and toxicology of cannabis: clinical implications.</a> Iran J Psychiatry. 7 (4), 149-156 (2012).</li>
<li>Metna-Laurent, M., Mondésir, M., Grel, A., Vallée, M., Piazza, P. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabinoid-Induced+Tetrad+in+Mice%5BTitle%5D)+AND+%22Curr+Protoc+Neurosci%22%5BJournal%5D)">Cannabinoid-Induced Tetrad in Mice.</a> Curr Protoc Neurosci. 80, 9.59.1-9.59.10 (2017).</li>
<li>Soo, J. C., Monaghan, K., Lee, T., Kashon, M., Harper, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Air+sampling+filtration+media%3A+Collection+efficiency+for+respirable+size-selective+sampling%5BTitle%5D)+AND+%22Aerosol+Sci+Technol%22%5BJournal%5D)">Air sampling filtration media: Collection efficiency for respirable size-selective sampling.</a> Aerosol Sci Technol. 50 (1), 76-87 (2016).</li>
<li>Tang, Z. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cytotoxicity+and+toxicoproteomic+analyses+of+human+lung+epithelial+cells+exposed+to+extracts+of+atmospheric+particulate+matters+on+PTFE+filters+using+acetone+and+water%5BTitle%5D)+AND+%22Ecotoxicol+Environ+Safety%22%5BJournal%5D)">Cytotoxicity and toxicoproteomic analyses of human lung epithelial cells exposed to extracts of atmospheric particulate matters on PTFE filters using acetone and water.</a> Ecotoxicol Environ Safety. 191, 110223(2020).</li>
<li>Puah, P. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Extractable+impurities+from+fluoropolymer-based+membrane+filters+-+interference+in+high-throughput%2C+untargeted+analysis%5BTitle%5D)+AND+%22RSC+Adv%22%5BJournal%5D)">Extractable impurities from fluoropolymer-based membrane filters - interference in high-throughput, untargeted analysis.</a> RSC Adv. 9 (55), 31918-31927 (2019).</li>
<li>Berrada-Gomez, M. P., Bui, B., Bondarenko, H., Ferret, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Particle+size+distribution+in+the+evaluation+of+the+inhalation+toxicity+of+cosmetic+spray+products%5BTitle%5D)+AND+%22Reg+Toxicol+Pharmacol%22%5BJournal%5D)">Particle size distribution in the evaluation of the inhalation toxicity of cosmetic spray products.</a> Reg Toxicol Pharmacol. 139, 105359(2023).</li>
<li>Hammer, T., Gao, H., Pan, Z., Wang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Relationship+between+Aerosols+Exposure+and+Lung+Deposition+Dose%5BTitle%5D)+AND+%22Aerosol+Air+Quality+Res%22%5BJournal%5D)">Relationship between Aerosols Exposure and Lung Deposition Dose.</a> Aerosol Air Quality Res. 20 (5), 1083-1093 (2020).</li>
<li>Asgharian, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Computational+modeling+of+nanoscale+and+microscale+particle+deposition%2C+retention+and+dosimetry+in+the+mouse+respiratory+tract%5BTitle%5D)+AND+%22Inhal+Toxicol%22%5BJournal%5D)">Computational modeling of nanoscale and microscale particle deposition, retention and dosimetry in the mouse respiratory tract.</a> Inhal Toxicol. 26 (14), 829-842 (2014).</li>
<li>Chou, L. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Particle+size+matters%3A+Discrepancies+in+the+health+risks+posed+by+traditional+cigarettes+and+e-cigarettes+in+mice+and+humans%5BTitle%5D)+AND+%22J+Hazardous+Mater+Lett%22%5BJournal%5D)">Particle size matters: Discrepancies in the health risks posed by traditional cigarettes and e-cigarettes in mice and humans.</a> J Hazardous Mater Lett. 4, 100088(2023).</li>
<li>Cheng, Y. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exposing+Animals+to+Oxidant+Gases%5BTitle%5D)+AND+%22Proc+Am+Thorac+Society%22%5BJournal%5D)">Exposing Animals to Oxidant Gases.</a> Proc Am Thorac Society. 7 (4), 264-268 (2010).</li>
<li>Chen, L. C., Lippmann, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inhalation+Toxicology+Methods%3A+The+Generation+and+Characterization+of+Exposure+Atmospheres+and+Inhalational+Exposures%5BTitle%5D)+AND+%22Curr+Protoc+Toxicol%22%5BJournal%5D)">Inhalation Toxicology Methods: The Generation and Characterization of Exposure Atmospheres and Inhalational Exposures.</a> Curr Protoc Toxicol. 63 (1), 24.24.21-24.24.23 (2015).</li>
<li>Wong, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inhalation+Exposure+Systems%3A+Design%2C+Methods+and+Operation%5BTitle%5D)+AND+%22Toxicologic+Pathol%22%5BJournal%5D)">Inhalation Exposure Systems: Design, Methods and Operation.</a> Toxicologic Pathol. 35 (1), 3-14 (2007).</li>
<li>Cousijn, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Grey+matter+alterations+associated+with+cannabis+use%3A+results+of+a+VBM+study+in+heavy+cannabis+users+and+healthy+controls%5BTitle%5D)+AND+%22Neuroimage%22%5BJournal%5D)">Grey matter alterations associated with cannabis use: results of a VBM study in heavy cannabis users and healthy controls.</a> Neuroimage. 59 (4), 3845-3851 (2012).</li>
<li>Filbey, F. M., Schacht, J. P., Myers, U. S., Chavez, R. S., Hutchison, K. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Marijuana+craving+in+the+brain%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Marijuana craving in the brain.</a> Proc Natl Acad Sci U S A. 106 (31), 13016-13021 (2009).</li>
<li>Sneider, J. T., Gruber, S. A., Rogowska, J., Silveri, M. M., Yurgelun-Todd, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+preliminary+study+of+functional+brain+activation+among+marijuana+users+during+performance+of+a+virtual+water+maze+task%5BTitle%5D)+AND+%22J+Addict%22%5BJournal%5D)">A preliminary study of functional brain activation among marijuana users during performance of a virtual water maze task.</a> J Addict. 2013, 461029(2013).</li>
<li>Gilman, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabis+use+is+quantitatively+associated+with+nucleus+accumbens+and+amygdala+abnormalities+in+young+adult+recreational+users%5BTitle%5D)+AND+%22J+Neurosci%22%5BJournal%5D)">Cannabis use is quantitatively associated with nucleus accumbens and amygdala abnormalities in young adult recreational users.</a> J Neurosci. 34 (16), 5529-5538 (2014).</li>
</ol>

<table><tbody><tr><td>2,2,2-Tribromoetanolo</td><td>Sigma-Aldrich</td><td>T48402-5G</td><td>Avertin</td></tr><tr><td>inExpose</td><td>SCIREQ</td><td>sales@scireq.com</td><td>www.scireq.com</td></tr><tr><td>Microtainer Serum Separator Tubes BD</td><td></td><td>365967</td><td></td></tr><tr><td>Pax Era Vape Pen</td><td>PAX</td><td></td><td>Acquistato da Ontario Cannabis Store</td></tr><tr><td>Pineapple Express Pax</td><td>Pod Buona fornitura</td><td></td><td>Acquistato da Ontario Cannabis Store</td></tr><tr><td>SoftRestraints</td><td>SCIREQ</td><td>IX-XN1-SR-AL</td><td>www.scireq.com</td></tr><tr><td>THC Forensic ELISA Kit</td><td>Neogen</td><td>131019</td><td></td></tr></tbody></table>

sviluppo di un modello di inalazione preclinica per testare i distillati di cannabis vaporizzati

Development of a Preclinical Inhalation Model to Test Vaporized Cannabis Distillates

L'obiettivo iniziale era quello di determinare un regime di esposizione in grado di fornire livelli fisiologicamente rilevanti di THC nel sangue rispetto agli esseri umani. Pertanto, una componente chiave per la selezione dei parametri di esposizione è stata l'utilizzo di caratteristiche che imitassero i modelli di utilizzo umano20,21. I topi maschi e femmine C57BL/6 sono stati esposti a un Pineapple Express Pax Era Pod contenente...

Sviluppo di un modello di inalazione preclinica per testare i distillati di cannabis vaporizzati

Despite their growing popularity, cannabis vape products remain understudied. Cannabis vape cartridges are used with battery-powered devices that ...

development-preclinical-inhalation-model-to-test-vaporized-cannabis

Research

Ricerca

JoVE Journal

Medicine

Medicina

Questo protocollo descrive un metodo per esporre i roditori al vapore elettronico di vapore (vapore E) e al fumo di sigaretta. Le camere di esposizione sono costruite modificando le camere di anestesia con un sistema di pompaggio automatico che fornisce vapori e fumo di sigaretta ai roditori. Questo sistema può essere facilmente modificato per ospitare molti endpoint sperimentali.

Confronto degli effetti di fumo elettronico di sigarette e sigaretta in un romanzo<em&gt; In Vivo</em&gt; Sistema di esposizione

Qui, descriviamo un dispositivo di laboratorio programmabile che può essere utilizzato per creare estratti di aerosol di sigaretta elettronica e fumo di sigaretta convenzionale. Questo metodo fornisce un utile strumento per effettuare confronti diretti tra sigarette convenzionali e sigarette elettroniche ed è un punto di ingresso accessibile in ricerca di sigaretta elettronica.

Un microcontrollore azionato il dispositivo per la generazione di estratti liquidi da Aerosol elettronico e fumo di sigaretta sigaretta convenzionale

Gli utenti di sigaretta elettronica (e-cig) sono in aumento in tutto il mondo. Piccolo, tuttavia, è conosciuto circa gli effetti di salute indotti dagli aerosol inalato di e-cig. Questo articolo descrive una tecnica di generazione dell'aerosol di e-cig adatta per esposizioni animali e successivi studi tossicologici. Tali protocolli sono tenuti a istituire sistemi di esposizione sperimentalmente riproducibile e standardizzato di e-cig.

Generazione di Aerosol di sigaretta elettronica da un dispositivo di macchina-Vaping di terza generazione: applicazione agli studi tossicologici

Presentiamo un sistema di esposizione in vitro robusto, trasferibile e predittivo per lo screening e il monitoraggio delle particelle trasportate dall'aria sulla loro citotossicità polmonare acuta esponendo le cellule polmonari umane coltivate all'interfaccia aria-liquido (ALI).

Valutazione della tossicità dell'inalazione acuta delle particelle aerotrasportate esponendo cellule polmonari umane coltivate all'interfaccia aria-liquida

Il protocollo descrive le impostazioni e l'uso di un generatore di aerosol capillare per la produzione continua di aerosol controllato da una soluzione liquida multispecie, adatta per la somministrazione costante di aerosol di grandi volumi (ad esempio, studi di inalazione in vivo ).

Uso del generatore di aerosol capillare nella produzione continua di aerosol controllato per studi non clinici

Il presente protocollo fornisce informazioni didattiche per l'uso del hornworm del tabacco Manduca sexta nella ricerca sui cannabinoidi.  Il metodo qui descritto include tutte le forniture e i protocolli necessari per monitorare i cambiamenti fisiologici e comportamentali del modello di insetto in risposta al trattamento con cannabidiolo (CBD).

Hornworm del tabacco come sistema modello di insetto per studi pre-clinici sui cannabinoidi

Stabiliamo un modello standardizzato in vitro per lo studio dell'esposizione ai vapori di cannabis all'interfaccia aria-liquido. Questo modello fornisce un approccio sistematico per esaminare gli impatti della cannabis vaporizzata, affrontando il crescente interesse per i vaporizzatori di cannabis come alternativa al fumo.

In vitro Esposizioni alla cannabis delle cellule epiteliali polmonari all'interfaccia aria-liquido

La somministrazione orale tramite sonda gastrica nei roditori può indurre angoscia associata alla contenzione. Abbiamo esplorato la somministrazione oromucosa/buccale di estratto di cannabis arricchito con CBD, in cui i ratti sono stati trattati per 15 giorni con l'estratto a 3 mg/kg/giorno. I trattamenti sono stati somministrati per via orale con una micropipetta, utilizzando una tecnica di tocco affettuoso per maneggiare gli animali.

Mucosa orale come metodo alternativo per la somministrazione di prodotti a base di cannabis nei roditori

Poiché le restrizioni legislative cambiano, con conseguente aumento dell'accessibilità dei prodotti a base di cannabinoidi, è di fondamentale importanza sviluppare modelli per studiare l'impatto di queste esposizioni, in particolare durante la gravidanza. È stato sviluppato un modello preclinico di esposizione prenatale moderata ai cannabinoidi attraverso l'ingestione volontaria, che consente indagini più approfondite.

Modello preclinico di esposizione prenatale al delta-9-tetraidrocannabinolo per valutarne l'impatto sugli esiti dello sviluppo neurologico

Il Δ 9-tetraidrocannabinolo (THC) è il costituente inebriante della cannabis e una sostanza controllata negli Stati Uniti. Questo articolo delinea le procedure standard per ottenere, conservare e somministrare il THC ai topi in conformità con le attuali normative legali e introduce brevemente un metodo per valutare gli effetti farmacodinamici di base del THC nei roditori.

Somministrazione di δ 9-tetraidrocannabinolo (THC) in topi adolescenti e adulti

Scopri come sviluppare un modello murino di inalazione per studiare gli effetti dei distillati di cannabis vaporizzati sulla funzione polmonare. Questo metodo consente di valutare le alterazioni trascrizionali nelle cellule epiteliali e i livelli di THC-COOH nel siero.

Sviluppo di un modello di inalazione preclinica per testare i distillati di cannabis vaporizzati

Esplora altri video