Hydrophobic Microbioreactor System
Hydrophobic Microbioreactor System - Related Videos
07:45
Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation
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Cited by 10 •
2020
Presented here are two protocols for the encapsulation of porcine oocytes in 3D culture conditions. In the first, cumulus-oocyte complexes (COCs) are encapsulated in fibrin-alginate beads. In the second, they are enclosed with fluorinated ethylene propylene powder particles (microbioreactors). Both systems ensure optimal conditions to maintain their 3D organization.
06:24
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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Cited by 9 •
2017
This manuscript describes a generic approach for tailor-made design of microbial cultivation media. This is enabled by an iterative workflow combining Kriging-based experimental design and microbioreactor technology for sufficient cultivation throughput, which is supported by lab robotics to increase reliability and speed in liquid handling media preparation.
02:51
Monitoring Bacterial Growth in Distinct Media Formulations with an Automated Microbioreactor
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2025
Source: Hemmerich, J., et al. Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology. J. Vis. Exp. (2017).This video demonstrates the use of an automated microbioreactor to monitor bacterial growth and fluorescent protein secretion in response to varying media formulations. A genetically modified bacterium expressing a secretory fluorescent protein is cultured under antibiotic selection and inoculated into nutrient-varied wells. Real-time optical...
03:22
Calcium-Dependent Hydrophobic Interaction Chromatography: A Technique to Purify Calcium-Binding Proteins Based on Hydrophobic Interactions
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2025
In this video, we demonstrate the purification of calcium-binding protein from a dialyzed cell lysate through calcium-dependent hydrophobic interaction chromatography. The calcium-binding proteins expose a hydrophobic region upon binding with calcium, facilitating interaction with a hydrophobic group on resin. Later these proteins are eluted using calcium chelator EDTA that reverses the interaction.
03:46
Butyl-Dependant Hydrophobic Interaction Liquid Chromatography: A Technique to Characterize Antibody-Drug Conjugates Based on Hydrophobic Interactions
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2025
This video demonstrates the hydrophobic interaction liquid chromatography-based characterization of antibody-drug conjugates. Using a butyl ligand-based chromatography column, the antibody-drug conjugates are characterized via hydrophobic interactions with the ligand.
FAQs
The water-repellent surfaces reduce wetting and unwanted microbial adhesion, helping keep aqueous media within designated microchambers or droplets. This confinement supports more consistent control of where cultures reside while preserving access to fluid handling and exchange functions during cultivation. As a result, researchers can better distinguish effects of selected experimental conditions across small-scale reactions.
These functions regulate the environment surrounding the microorganisms. Fluid handling controls the movement or placement of culture media, gas exchange supports the selected cultivation conditions, and nutrient delivery supplies resources needed for growth. Their coordination allows researchers to adjust experimental conditions and observe how microorganisms respond through changes in growth, physiology, or product formation.
Small reaction volumes reduce material use while allowing many experiments to run in parallel. Researchers can therefore compare selected cultivation conditions, microbial strains, or treatment settings more efficiently than in a single larger reaction. The resulting high experimental throughput is particularly valuable when screening alternatives or optimizing early-stage biological processes.
Researchers place aqueous culture media in defined microchambers or droplets, establish the desired fluid-handling and environmental conditions, and allow microorganisms to grow under those settings. Measurements can then be collected across multiple reactions to compare growth kinetics, physiology, or product formation. This workflow links controlled cultivation with rapid, parallel analysis.
The platform supports strain screening, antimicrobial testing, and bioprocess optimization. In strain screening, parallel reactions can help compare microbial performance. For antimicrobial studies, researchers can examine responses under selected conditions. During bioprocess optimization, the system helps evaluate cultivation settings and product formation while using relatively small amounts of material.
Measurements from the platform can reveal microbial growth kinetics, meaning how growth changes over time, along with aspects of cellular physiology and product formation. These outcomes help researchers compare strains or conditions, identify promising experimental settings, and guide early-stage process development. The same measurements also connect microscale cultivation with broader biology research questions.