Cardiac Tissue Slices
Cardiac Tissue Slices - Related Videos
04:58
Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue
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2025
This video demonstrates a vibratome-based procedure to generate viable, ultra-thin sections of pig heart. The prepared tissue slices can be cultured and used as model systems for pharmacological testing.
04:40
Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting
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Cited by 55 •
2017
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
10:09
Capillary Force Lithography for Cardiac Tissue Engineering
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Cited by 27 •
2014
In this protocol, we demonstrate the fabrication of biomimetic cardiac cell culture substrata made from two distinct polymeric materials using capillary force lithography. The described methods provide a scalable, cost-effective technique to engineer the structure and function of macroscopic cardiac tissues for in vitro and in vivo applications.
03:40
TTC Staining of Rat Brain Tissue Slices: A Staining Procedure to Differentiate Viable and Necrotic Tissue in Tissue Slices After Ischemia-Reperfusion Injury
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2025
This video describes the 2,3,5-triphenyl-2H-tetrazolium chloride staining procedure to visualize rat brain tissue slices and differentiate viable and damaged tissue following middle cerebral artery occlusion and reperfusion.
05:02
Cerebellum Dissection and Slice Preparation: A Method to Generate Tissue Slices from Mouse Cerebellum
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2025
This video describes a method to generate tissue slices from the mouse cerebellum. The cerebellar slices can be used for organotypic culturing that provides insights into neurodevelopmental mechanisms, including myelination dynamics.
FAQs
Maintaining native architecture keeps cardiomyocytes, connective tissue, and vascular components organized and interacting within the same preparation. This arrangement allows researchers to examine cardiac responses in a setting that retains relationships lost when cells are studied separately. As a result, experimental observations can reflect coordinated tissue behavior rather than the activity of isolated cardiomyocytes alone.
Cardiac tissue slices support analysis of several connected biological processes, including electrophysiology, contraction, and metabolism. They can also be used to investigate responses associated with injury and repair. Because experimental stimuli or compounds can be applied while tissue organization remains intact, researchers can examine how these functions change together under controlled ex vivo conditions.
They provide an intermediate experimental scale between isolated cells and whole-animal or human heart studies. Unlike isolated cells, the slices preserve tissue organization and interactions among multiple cardiac components. Unlike whole-organism experiments, they allow controlled application of stimuli or compounds directly to the preparation, helping researchers study cardiac responses while reducing the complexity of a complete organism.
Researchers prepare thin sections from heart tissue and maintain them under controlled culture conditions. The goal is to keep the native organization of cardiomyocytes, connective tissue, and vascular components available for study ex vivo. Once maintained, the slices can receive defined experimental stimuli or compounds, enabling observations of cardiac behavior within an organized tissue context.
The preparation allows researchers to apply experimental compounds or other stimuli while monitoring relevant cardiac outcomes. Depending on the study, these outcomes may include electrophysiological behavior, contraction, metabolism, injury responses, or repair-related processes. This controlled exposure makes the model useful for examining how cardiac tissue reacts to candidate interventions or disease-associated conditions.
They provide a physiologically relevant platform for evaluating drug effects and investigating disease mechanisms. Researchers can observe compound-associated changes in organized cardiac tissue rather than relying only on isolated cellular responses. The same approach supports studies of injury and repair, helping connect molecular or cellular effects with broader changes in cardiac tissue function.