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JoVE Journal
Bioengineering

This content is Open Access.

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
 

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Article DOI: 10.3791/56234
December 15th, 2017

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Summary December 15th, 2017

This manuscript describes a generic approach for tailor-made design of microbial cultivation media. This is enabled by an iterative workflow combining Kriging-based experimental design and microbioreactor technology for sufficient cultivation throughput, which is supported by lab robotics to increase reliability and speed in liquid handling media preparation.

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