Matrigel Injection
Matrigel Injection - Related Videos
03:50
From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
0 Views •
Cited by 3 •
2008
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 3 of 3.
06:40
From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
0 Views •
Cited by 4 •
2008
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 2 of 3.
06:29
From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs
0 Views •
Cited by 6 •
2008
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells. Part 1 of 3.
04:00
A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells
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Cited by 121 •
2011
A tube formation assay is used to evaluate vascular activity of tumor cells.
08:28
Zebrafish Brain Ventricle Injection
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Cited by 72 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
FAQs
Matrigel should be maintained cold until injection because warming at physiological temperature triggers polymerization into a three-dimensional gel. This transition changes the material from a liquid suitable for delivery into a matrix that supplies structural and biochemical cues during the experiment, helping establish a controlled environment for the administered cells or biological material.
They can change the observed behavior and reproducibility of a model. Matrix composition and injection site influence the local context, while cell type affects how tumor cells respond within that context. Consequently, measurements of tumor growth, invasion, angiogenesis, or tissue interactions may differ across experiments and should be interpreted alongside these experimental variables.
Once polymerized, the matrix creates a three-dimensional setting rather than merely transporting material. That setting supports controlled examination of tumor growth, invasion, angiogenesis, and interactions with surrounding tissues. Its structural and biochemical cues help connect the injected material to tissue-level behavior, making it useful for studying how cancer cells develop within a localized environment.
The workflow begins with keeping Matrigel cold, suspending the selected cells, drug, or biological material in it, and delivering that suspension by injection to the chosen site. As the preparation warms to physiological temperature, it polymerizes, establishing the localized three-dimensional environment used for subsequent cancer or tissue studies.
Investigators can use it to establish localized tumor models and examine tumor growth, invasion, angiogenesis, and interactions with surrounding tissues. The approach also supports drug-response testing and xenograft studies. Its value lies in combining a defined delivery site with matrix-associated cues, allowing researchers to examine cancer progression or treatment effects under controlled experimental conditions.
Differences may reflect more than the injected cancer cells or treatment. Matrix composition, injection site, cell type, and other experimental conditions can all influence the outcome. Researchers should therefore consider these factors when comparing tumor growth, invasion, angiogenesis, tissue interactions, or drug responses, because the method does not produce identical results across every model.