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From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
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Biology
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JoVE Journal Biology
From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

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03:50 min

June 10, 2008

DOI:

03:50 min
June 10, 2008

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Summary

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This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 3 of 3.

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