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From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
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From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

DOI:
10.3791/831-v

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06:40 min

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June 09, 2008

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, ,
1David Geffen School of Medicine,University of California, Los Angeles

Chapters

  • 00:00Introduction
  • 00:26hESC Culture Daily Maintenance
  • 01:50Splitting hESCs onto Matrigel
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 2 of 3.

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MEFsMatrigelHESCsFeeder CellsPassageGrowHuman Embryonic Stem Cells

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