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From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
JoVE Journal
Biology
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JoVE Journal Biology
From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

11,396 Views

06:40 min

June 09, 2008

DOI:

06:40 min
June 09, 2008

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Summary

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This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 2 of 3.

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