This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 2 of 3.
Khvorostov, I., Zhang, J., Teitell, M. From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel. J. Vis. Exp. (16), e831, doi:10.3791/831 (2008).