Biology
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From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
Chapters
Summary June 9th, 2008
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 2 of 3.
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