Spleen Deficiency

Spleen deficiency is a diagnostic pattern in Traditional Chinese Medicine (TCM), describing impaired digestive and energy-transforming functions attributed to the Spleen rather than a confirmed biomedical disease. TCM theory links the pattern to reduced transformation and transportation of food and fluids, which may present with fatigue, poor appetite, bloating, loose stools, or a heavy sensation. Practitioners assess these findings alongside the tongue and pulse to distinguish Spleen Qi, Yang, or related deficiency patterns. In clinical practice, this framework guides individualized treatment plans involving dietary recommendations, acupuncture, moxibustion, or herbal medicine, while biomedical evaluation remains important for identifying underlying conditions.

Spleen Deficiency - Related Videos

Research

JoVE EoE - Immunotherapy

A Mouse Model of Vascularized Heterotopic Spleen Transplantation

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2025

The video demonstrates a surgical method for generating a mouse model of vascularized heterotopic spleen transplantation. The abdominal cavity of an anesthetized donor mouse is surgically opened to remove the spleen. The inferior vena cava and infrarenal aorta are identified, their lumbar branches are ligated, and openings are made for the heterotopic transplantation of a spleen from a congenic donor mouse. Upon completing vascular anastomosis and restoring blood flow to the implanted spleen,...

Assessing Bacterial Burden in the Mouse Spleen for Infection Studies

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2026

Source: Madenspacher, J. H. and Fessler, M. B. A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice. J. Vis. Exp. (2016)This video demonstrates the process of quantifying bacterial load in the spleen of an infected mouse to assess the severity of infection. The homogenized spleen is serially diluted and plated to determine total bacterial burden. The resulting data provide insights into host-pathogen interactions within...

Research

JoVE Journal - Biology
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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

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Cited by 22 •

2008

This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.

Quantification of Bacterial Load in Mouse Spleen and Liver After Systemic Infection

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2026

Source: Ahn, J. J., et al. Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses. J. Vis. Exp. (2016)The video demonstrates bacterial load measurement in spleen and liver tissues isolated from mice infected with an intracellular bacterial pathogen. Bacteria, accumulated within tissue-resident phagocytes during systemic infection, are released using detergent-based lysis and mechanical disruption. Serial dilutions of the homogenates are plated on...

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

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Cited by 17 •

2012

We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.

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