Human Fetal Rpe
Human Fetal Rpe - Related Videos
06:54
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
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Cited by 7 •
2017
The human retina is composed of functionally and molecularly distinct regions, including the fovea, macula, and peripheral retina. Here, we describe a method using punch biopsies and manual removal of tissue layers from a human eye to dissect and collect these distinct retinal regions for downstream proteomic analysis.
10:19
MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
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Cited by 10 •
2014
The microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, retinal pigment epithelium (RPE) derived from human induced-pluripotent stem (iPS) cells (iPS-RPE), and fetal RPE, were compared.
08:19
Isolation of Leukocytes from the Human Maternal-fetal Interface
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Cited by 57 •
2015
Described herein is a protocol to isolate and further study the infiltrating leukocytes of the decidua basalis and decidua parietalis - the human maternal-fetal interface. This protocol maintains the integrity of cell surface markers and yields enough viable cells for downstream applications as proven by flow cytometry analysis.
14:26
Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain
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Cited by 38 •
2012
Primary, human fetal brain-derived, multipotential progenitor cells proliferate in vitro while maintaining the capacity to differentiate into neurons and astrocytes. This work shows that neural progenitors can be induced to differentiate through stages of the oligodendrocytic lineage by conditioning with select growth factors.
07:29
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
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Cited by 19 •
2011
A simple and reliable method on isolation and culture of neural stem cells from discarded human fetal cortical tissue is described. Cultures derived from known human neurological disorders can be used for characterization of pathological cellular and molecular processes, as well as provide a platform to assess pharmacological efficacy.
FAQs
Human fetal RPE supports retinal homeostasis through two linked activities: maintaining the blood-retina barrier and transporting nutrients. The barrier helps regulate exchange around neural retinal tissue, while nutrient transport supports the cells required for development and visual function. Studying these activities helps neuroscience researchers connect RPE physiology with the formation and maintenance of the developing retina.
The retinoid cycle allows RPE cells to recycle visual pigments, linking epithelial activity with the retina’s ability to support visual function. Examining this process in human fetal RPE can help researchers investigate how pigment recycling contributes to retinal development and how disruption of RPE support may affect photoreceptors or other neural retinal components.
Phagocytosis of shed photoreceptor outer segments is a maintenance process that helps preserve photoreceptor health. In human fetal RPE studies, this activity provides a way to examine communication between supporting epithelial cells and photoreceptors, while also relating cellular waste removal to the long-term preservation of retinal tissue and visual function.
Its developmental properties make human fetal RPE a model for examining how RPE cells interact with the developing neural retina. Researchers can use this context to study cell-cell signaling during retinal formation and to identify how support from pigmented epithelial cells contributes to the emergence and maintenance of visual function.
Human fetal RPE can support studies of mechanisms involved in degenerative disease, particularly when retinal or RPE support is lost. Because the cells participate in barrier maintenance, nutrient transport, pigment recycling, and photoreceptor maintenance, they provide a research context for connecting failures in these functions with damage to visual tissues.
Human fetal RPE contributes to research on RPE replacement, retinal repair, and potential cell-based therapies. Its developmental characteristics help researchers consider how replacement cells might restore support for damaged retinal tissue. This work is relevant to conditions involving photoreceptor or RPE loss, where preserving or rebuilding the supporting environment may be central to recovery.