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Biochemistry
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JoVE Journal Biochemistry
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
 

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

Article doi: 10.3791/56203
November 12th, 2017 • Usage Statistics
*1,2,7,8, *3,4, 3,4, 1,2, 3, 3, 1,2, 5,6, 3,9
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, 2Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, 4Medical Scientist Training Program, University of Iowa, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7Department of Ophthalmology, Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology, Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
* These authors contributed equally

Summary
Automatic Translation
November 12th, 2017

The human retina is composed of functionally and molecularly distinct regions, including the fovea, macula, and peripheral retina. Here, we describe a method using punch biopsies and manual removal of tissue layers from a human eye to dissect and collect these distinct retinal regions for downstream proteomic analysis.

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