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Chapters
- 00:05Title
- 02:20Initiating Differentiation of Neural Progenitor Cells into Progenitor-derived Oligodendrocytes
- 05:45Flow Cytometry Assay
- 09:29Immunofluorescence Microscopy
- 11:22Representative Results
- 13:54Conclusion
Primary, human fetal brain-derived, multipotential progenitor cells proliferate in vitro while maintaining the capacity to differentiate into neurons and astrocytes. This work shows that neural progenitors can be induced to differentiate through stages of the oligodendrocytic lineage by conditioning with select growth factors.
Tags
Progenitor-derived Oligodendrocyte Culture SystemHuman Fetal BrainDifferentiationNeural ProgenitorsNeuronal Cell TypesGlial Cell TypesMolecular RegulationNeural Cell Lineage DevelopmentIn Vitro ExpansionGlial ProgenitorsSub-cortical White MatterCulture ConditionsMyelin Producing OligodendrocytesGalactocerebroside+O4+ Oligodendrocyte Precursor Or Progenitor Cells (OPC)Neural Precursor CellsSupport Cells (astrocytes And Neurons)Culture SystemIn Vitro ExperimentationPrimary Human OligodendrocytesPathogenesisNeurotropic Infectious Agents (human PolyomavirusJCV)Demyelinating DiseasesCentral Nervous System (CNS)
