細胞信号を解析する統合ツールキット:力、運動、形態、蛍光

5.2K 回視聴

7 件の引用

14:55 分

2022年3月5日

10.3791/63095-v

2022年3月5日

5.2K 回視聴

Alyson Nguyen^*¹ , Keith Battle^*²^,³ , Sunita S. Paudel^*²^,³ , Ningyong Xu² , Jessica Bell³ , Linn Ayers³ , Cassandra Chapman⁴ , Ajay P. Singh⁵ , Srinivas Palanki⁶ , Thomas Rich³^,⁷ , Diego F. Alvarez⁸ , Troy Stevens²^,³ , Dhananjay T. Tambe³^,⁴^,⁷

¹Biomedical Sciences, Pat Capps Covey College of Allied Health Professions, University of South Alabama, ²Department of Physiology and Cell Biology, College of Medicine, University of South Alabama, ³Center for Lung Biology, College of Medicine, University of South Alabama, ⁴William B. Burnsed Jr. Mechanical, Aerospace, and Biomedical Engineering Department, College of Engineering, University of South Alabama, ⁵Mitchell Cancer Institute, College of Medicine, University of South Alabama, ⁶Department of Chemical and Biomedical Engineering, West Virginia University, ⁷Department of Pharmacology, College of Medicine, University of South Alabama, ⁸Department of Physiology & Pharmacology, College of Osteopathic Medicine, Sam Houston State University

^* These authors contributed equally

細胞信号を分析する統合ツールキット(iTACS)プラットフォームは、接着細胞内の多種多様な化学および機械的信号を同時に測定するプロセスを自動化します。iTACSは、コミュニティ主導の開発を容易にし、研究者が学歴に関係なくすべてのプラットフォーム機能を使用できるように設計されています。

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Chapters in this video

0:02

Introduction

1:46

Creation of Position List

3:57

Automated Image Acquisition for the Remaining Experiment

4:34

Setting Up Automated Data Analysis

6:08

Quantification of Hydrogel Deformation

6:37

Quantification of Cell-ECM and Cell-cell Forces

8:44

Segmentation of the Individual Cells in the Images

9:47

Mapping of the Pixel Intensities on the Cells

11:06

Mapping of the Forces and Motion on the Cells

11:51

Tracking of the Cellular Indentities across the Experiment

12:51

Generation of Time Tracks of the Assessed Cellular Properties

13:27

Generation of the Heat Maps of the Assessed Cellular Properties

13:53

Representative Results

14:30

Conclusion

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