Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

Anna E. Tseng; Aoife O'Connell; Aaron Benhamou; Shivraj Yabaji; Igor Kramnik; Nicholas A. Crossland; Emily Speranza

doi:10.3791/67853

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

反復漂白は、コア施設の染色自動化を使用して多様性を拡張します

Full Text

641 Views

04:52 min

August 6, 2025

DOI: 10.3791/67853-v

Anna E. Tseng^1,2, Aoife O'Connell¹, Aaron Benhamou^1,3, Shivraj Yabaji^1,2, Igor Kramnik^1,2, Nicholas A. Crossland^1,2,3, Emily Speranza⁴

¹National Emerging Infectious Diseases Laboratories (NEIDL),Boston University, ²Department of Pathology and Laboratory Medicine,Boston University Chobanian and Avedisian School of Medicine, ³Department of Virology, Immunology and Microbiology,Boston University Chobanian and Avedisian School of Medicine, ⁴Florida Research and Innovation Center,Cleveland Clinic Foundation

Please note that some of the translations on this page are AI generated. Click here for the English version.

ここでは、市販の自動組織染色装置を利用した反復漂白拡張多重度(IBEX)法の適用と、チラミドシグナル増幅ベースの試薬の使用に関する最適化の考慮事項について説明します。

Explore More Videos

免疫学と感染症第222号