Intact Drg Preparation
Intact Drg Preparation - Related Videos
09:14
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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Cited by 20 •
2011
The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.
03:44
Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane
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2025
The video demonstrates the co-culturing of dorsal root ganglion (DRG) neurons with Schwann cells. DRG neurons are first grown on a pre-stretched membrane for aligned axon growth. Schwann cells are then introduced, which attach to the axons, insulating them and promoting neural cell survival and function.
12:00
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
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Cited by 8 •
2016
This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.
13:37
Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
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Cited by 34 •
2012
This protocol illustrates a harvesting technique for coccygeal bovine intervertebral discs for organ culture for in vitro organ culture.
04:25
Isolation of DRG Neurons and Coculture with Schwann Cell Precursors to Generate Schwann Cells
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2025
This video demonstrates a procedure for isolating dorsal root ganglion (DRG) neurons from rat embryos and co-culturing them with Schwann cell precursors to generate mature Schwann cells. The isolated DRGs are processed to isolate neurons and glial cells. The isolated cells are cultured to purify the neurons, which are then co-cultured with Schwann cell-like cells (SCLCs) in a co-culture medium. Interaction with the DRG neurons leads to the maturation of SCLCs into Schwann cells.
FAQs
Preserving the ganglion’s architecture keeps neuronal cell bodies, satellite glial cells, and local extracellular components in their spatial context. This allows investigators to examine protein expression, signaling, and metabolism while cellular interactions remain intact. Compared with dissociated-cell studies, the preparation can provide biochemical observations that better reflect tissue-level relationships involved in sensory nervous system function.
Neuronal cell bodies provide a primary source of sensory-system biochemical activity, while satellite glial cells and surrounding extracellular components contribute to the local signaling environment. Maintaining these elements together helps researchers assess responses that may depend on communication among cell types rather than on isolated cells alone. This is especially relevant when studying neuroinflammation or injury-related molecular changes.
Controlled ex vivo conditions help minimize structural and molecular disruption after isolation. That stability matters because handling-related changes could alter protein expression, signaling, or metabolic measurements and complicate interpretation. Maintaining the ganglion under consistent conditions therefore supports more reliable comparisons between untreated tissue and tissue exposed to an experimental treatment, while retaining a model that is more localized than a whole-animal study.
The preparation occupies an intermediate experimental scale. It preserves tissue-level organization that dissociated cells lack, yet focuses analysis on the dorsal root ganglion rather than the entire organism. Researchers can therefore examine sensory-system biochemistry and treatment responses within a defined tissue context, then compare those findings with cellular mechanisms from dissociated cultures or broader physiological effects observed in whole-animal studies.
The workflow begins with careful dissection from surrounding spinal tissue, followed by removal of connective tissue that could interfere with access or analysis. The isolated ganglion is then maintained under controlled ex vivo conditions to limit structural and molecular disruption. Each stage supports preservation of the tissue’s native organization, which is necessary for subsequent assessment of proteins, signaling, metabolism, or treatment responses.
Researchers may choose it when they need to examine biochemical events within organized sensory tissue rather than in isolated cells. The model supports studies of protein expression, cell signaling, metabolism, sensory processing, neuroinflammation, and nerve injury. It is also useful when an experimental treatment must be evaluated in the presence of neuronal, glial, and local extracellular interactions.
This preparation can reveal changes in protein expression, signaling activity, metabolism, and responses to experimental treatments within preserved sensory tissue. Such measurements may help connect molecular changes with processes involved in sensory processing, neuroinflammation, or nerve injury. Because the tissue architecture remains available for study, findings can add context that is difficult to obtain from isolated cellular measurements alone.