Salad Spinner

A salad spinner is a manually driven kitchen device that removes water from washed produce by rotating it inside a perforated basket. As the basket spins, centrifugal acceleration pushes surface water outward through the openings while the greens remain contained, illustrating how rotational motion can move liquid away from a solid material. In biochemistry education, this simple tool provides an accessible analogy for centrifugation and helps students distinguish mechanical dewatering from laboratory centrifugation, which separates suspended components according to properties such as size and density. It can also support discussions of sample preparation, equipment design, and controlled experimental conditions.

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Research

JoVE EoE - Neuronal Culture Techniques

An In Vitro Method to Generate Human Brain Organoids

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2025

The video demonstrates a method for generating human brain organoids from induced pluripotent stem cells. It shows how the neurosphere forms a neuroectodermal layer, which suppresses the formation of other germ layers. The neurospheres are embedded in a 3D gel matrix and form neuroepithelial buds. When incubated with organoid media, these neuroepithelial buds differentiate into specific brain regions, resulting in the formation of mature brain organoids.

Generation of Chikungunya Virus-Like Particles Using Baculovirus Expression System in Insect Cells

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2025

This video shows how virus-like particles, VLPs are generated by infecting insect cells with a recombinant baculovirus carrying the chikungunya virus gene cassette. The recombinant virus infects cells, leading to VLP formation, assembly, and release into the culture medium for potential use in vaccine production.

Large-Scale Production of High-Capacity Adenoviral Vectors

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2026

Source: Ehrke-Schulz, E., et al. Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5. J. Vis. Exp. (2016)This video demonstrates the large-scale amplification of high-capacity adenoviral vectors in suspension-adapted human embryonic kidney cells expressing Cre recombinase for use in gene delivery applications requiring high-titer, helper-free vector preparations.

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

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Cited by 46 •

2017

Modeling human brain development has been hindered due to the unprecedented complexity of neural epithelial tissue. Here, a method for the robust generation of brain organoids to delineate early events of human brain development and to model microcephaly in vitro is described.

Generating 3D Co-culture Spheres of Astrocytes and Neurons to Induce Synapse Formation

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2025

This video demonstrates a procedure for co-culturing the astrocytes and neurons to form 3D spheres. These 3D spheres provide a distinct platform for examining synapse formation and neural network integration, thereby serving as a potential model for research in developmental neuroscience, neurodegenerative diseases, and neuropharmacological interventions.

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