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Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

DOI:
10.3791/55372-v

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07:40 min

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April 14, 2017

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,
1Center for Molecular Medicine Cologne,University of Cologne, 2Institute for Biochemistry I,Medical School of University of Cologne

Chapters

  • 00:05Title
  • 00:49Embedding Neurospheres in EHS Matrix
  • 01:57Organoids in a Rotary Suspension Culture
  • 03:08Analysis of Brain Organoids
  • 05:47Results: Immunofluorescent Imaging of Cryosectioned Organoids
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

Modeling human brain development has been hindered due to the unprecedented complexity of neural epithelial tissue. Here, a method for the robust generation of brain organoids to delineate early events of human brain development and to model microcephaly in vitro is described.

Tags

Keywords: IPSCBrain OrganoidsNeurodevelopmental DisordersNeurogenesisPrimary MicrocephalyNeurospheresEHS MatrixSpinner FlaskBrain Organoid MediumOrganoid Culture

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