Dual-sided Illumination
Dual-sided Illumination - Related Videos
05:58
Surgical Trunk Oriented Laparoscopic Right Hemicolectomy (ST-LRH) for Right-Sided Colon Cancer
0 Views •
2025
This protocol describes Surgical Trunk-oriented Laparoscopic Right Hemicolectomy (ST-LRH), a modified laparoscopic right hemicolectomy designed to optimize vascular management and lymph node dissection, simplify surgical procedures, and improve surgical safety and efficiency.
03:22
Phototaxis Assay of Filamentous Cyanobacteria Using Focused Light Illumination
0 Views •
2025
Source: Weber, N. et. al., Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna. J. Vis. Exp. (2022)This video demonstrates a focused LED-based assay for studying phototaxis in filamentous cyanobacteria. The bacteria migrate toward the light source, forming a dense accumulation at the illuminated center, which is visualized and quantified through pixel intensity analysis of the captured image.
10:07
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
0 Views •
2014
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
11:35
Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
0 Views •
Cited by 16 •
2010
A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.
07:33
In vivo Dual Substrate Bioluminescent Imaging
0 Views •
Cited by 20 •
2011
Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
FAQs
Light entering from only one surface can lose intensity as it travels through absorbing or scattering material, leaving deeper regions less exposed. Illumination from the opposite surface provides an additional path for light to reach those regions. This reduces the effect of one-sided attenuation and shadowing, helping create a more even optical environment throughout the sample.
Absorption removes light as it passes through the sample, while scattering redirects it and can produce spatially uneven exposure. Differences in optical properties across a tissue construct or device increase this heterogeneity. By approaching the material from both surfaces, the method can reduce the extent to which any single region is governed by one difficult optical path.
One-sided illumination relies on light traveling through the sample from a single entry surface, so attenuation and shadowing accumulate along that direction. The dual-sided approach addresses the same material through opposing surfaces, reducing dependence on one path. This distinction matters when sample thickness or internal optical variation would otherwise create strong differences between exposed and deeper regions.
More uniform exposure can support optical stimulation, imaging, photochemical reactions, and other light-activated processes in three-dimensional biological systems. The benefit is not limited to one type of readout: improving light distribution can help the interior of a sample participate more consistently in the intended optical process. This makes the approach useful when uneven illumination limits performance.
The sample, tissue construct, or device is positioned so that illumination reaches it through two opposing surfaces rather than through only one face. The arrangement should preserve access to both sides of the material while maintaining the intended optical interaction with its interior. This basic geometry can be applied to three-dimensional systems where light penetration is a limiting factor.
It is useful when engineered tissues or organ-on-chip platforms have enough thickness or optical heterogeneity to make single-sided exposure uneven. In these settings, dual-sided illumination can improve access to interior regions during imaging, stimulation, or light-activated processing. Its relevance comes from addressing a shared bioengineering problem: delivering light more uniformly through three-dimensional biological constructs and devices.